Sequences from confirmation amplicon


Confirmation sequences for line 259D10 and hit At5g13400 are shown.
Line ID 259D10
Locus At5g13400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4298509
Primers used for PCR 07re - 8474, show primer details
   
Sequence Name 83-K115990-0022-259-D10C-07re
GenBank Accession LN514350 [GenBank]
Sequencing primer gene specific primer, 07re: 5'-ACTCAGCATTATAGTGCATGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K115990-0022-259-D10C-07re
TTCCTCTACTTGTGTACTGTGCATAGCTTCCATGGAGATGGTTCCAATCCATCTTCTTTC
ACTCAAGTGCCGCCTTGTCTAGCCATCTGCATATGAATTCATTCGACTGATAAATACTAG
TATTAAGTTTTATTTTCCTTATAGGAAGATGTACACAAAAGAAGAAGCCTTACATGAAAT
CATTGGAATGAGGGATTTTACGACTGCCATTAATAGCAGATTTCAAGCCAGGAACTTCGT
AAAGACCGATGAACTCACTGCTTGTGAAAGCTGCATTTCGTTTGCGGAAAGCGGCAACTA
AGACCTGAGCTACCCTAGTCAAGGGACTACCACCAGGCAACCTATGCCGATAAAGCGGTG
TACCAGCAAAGAACAAAGCATTAGAAATCCCCATGGCTACGGCCAATGTGCCAAAAGCCA
TACCCCAACCAAGTTCCATTTGAACATATACCACCAAAGTGAAGGCAATGATTGCGCCTA
AAGTGACCGATAGATAGAAGAAGTTGAAGAAACGGTCTAAATGTGTCTTGTAGTCTTTGC
TCTTCTCATCAAACTGGTCAGCACCAAATGAGGAAACACATGGCCTTATACCAGCAGCTC
CGAATCCGGTTATGTAGAGAACCGTGTAGAGATAAAGCATTTGCCAAGATTTGGCTTCTT
CACAATTTCCTAAGAGCAATGATAACTGGCCACAGTTGCTCTGGTCTGGTACGAACATCT
TCAAACTTGCCCCCAATGTTATTCCTATCANACCCTTTTCACATGCAAAACAAAAGAAAA
GAAAAGATTTCAAAGTATCCATCACATAACATATGTTCGGTCTTGCGTATTAGACGATGT
TCTTCAAATTGCACCATTTGCACAAGACCTTAACCATAAGTCATAAATAAATTGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAAT
   
Sequence Name 64-K115989-0022-259-D10C-8409
GenBank Accession LN511273 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K115989-0022-259-D10C-8409
CATTTATTTTGACTTATGGTTAAGGTCTTGTGCAAATGGTGCAATTTGAAGAACATCGTC
TAATACGCAAGACCGAACATATGTTATGTGATGGATACTTTGAAATCTTTTCTTTTCTTT
TGTTTTGCATGTGAAAAGGGTTTGATAGGAATAACATTGGGGGCAAGTTTGAAGATGTTC
GTACCAGACCAGAGCAACTGTGGCCAGTTATCATTGCTCTTAGGAAATTGTGAAGAAGCC
AAATCTTGGCAAATGCTTTATCTCTACACGGTTCTCTACATAACCGGATTCGGAGCTGCT
GGTATAAGGCCATGTGTTTCCTCATTTGGTGCTGACCAGTTTGATGAGAAGAGCAAAGAC
TACAAGACACATTTAGACCGTTTCTTCAACTTCTTCTATCTATCGGTCACTTTAGGCGCA
ATCATTGCCTTCACTTTGGTGGTATATGTTCAAATGGAACTTGGTTGGGGTATGGCTTTT
GGCACATTGGCCGTAGCCATGGGGATTTCTAATGCTTTGTTCTTTGCTGGTACACCGCTT
TATCGGCATAGGTTGCCTGGTGGTAGTCCCTTGACTAGGGTAGCTCAGGTCTTAGTTGCC
GCTTTCCGCAAACGAAATGCAGCTTTCACAAGCAGTGAGTTCATCGGTCTTTACGAAGTT
CCTGGCTTGAAATCTGCTATTAATGGCAGTCGTAAAATCCCTCATTCCAATGATTTCATG
TAAGGCTTCTTCTTTTGTGTACATCTTCCTATAAGGAAAATAAAACTTAATACTAGTATT
TATCAGTCGAATGAATTCATATGCAGATGGCTAGACAAGGCGGCACTTGAGTTGAAAGAA
GATGGATTGGAACCATCTCCATGGAAGCTATGCACAGTAACACAAGTAGAGGAAGTGAAG
ATTCTGATCAGATTGATTCCAATCCCAACATGC

Last Updated on 06.12.2018 16:11