Sequences from confirmation amplicon


Confirmation sequences for line 007C11 and hit At5g13550 are shown.
Line ID 007C11
Locus At5g13550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4359545
Primers used for PCR 23km - 8474, show primer details
   
Sequence Name 53-K116249-0022-007-C11C-23km
GenBank Accession LN508265 [GenBank]
Sequencing primer gene specific primer, 23km: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116249-0022-007-C11C-23km
CATTTCTGGTACCTATTAAATAAAGAATAGTAAACAAATCATCAAAACTAGCACGCAAGC
CACTTGTATTGGATTGACCCCTCATGAATTCTATTCTATTAATTAGAGATACGTGACGAA
ACTTACAAGTGACAACAATTTATCTAATTACTAATTAACAACTAATTTTAAAAATGTTAT
GTGATTAGCAAAATTTAAATGTAGTTACGTTATCGTAATTCTGGATCTATTACTATAAAC
TAAATTATTTTTGACGTATGATTAGATAATGTAATGATAATTGTAAAGTTTAGTTTGAAA
AAAAACAAAATTGACCAGACACTAGAGTAAAGCTTATGAGCATTGATGGAATAAATGATA
CAAAGCTCTTTAACATTTGAGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATAAAAAA
   
Sequence Name 29-K116248-0022-007-C11C-8409
GenBank Accession LN499440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116248-0022-007-C11C-8409
CATTTACATTGAATACTCAAATGTTAAAGAGCTTTGTATCATTTATTCCATCAATGCTCA
TAAGCTTTACTCTAGTGTCTGGTCAATTTTGTTTTTTTTCAAACTAAACTTTACAATTAT
CATTACATTATCTAATCATACGTCAAAAATAATTTAGTTTATAGTAATAGATCCAGAATT
ACGATAACGTAACTACATTTAAATTTTGCTAATCACATAACATTTTTAAAATTAGTTGTT
AATTAGTAATTAGATAAATTGTTGTCACTTGTAAGTTTCGTCACGTATCTCTAATTAATA
GAATAGAATTCATGAGGGGTCAATCCAATACAAGTGGCTTGCGTGCTAGTTTTGATGATT
TGTTTACTATTCTTTATTTTAATAGGTACCAGAAATTGGGAGGGAATAAAACATGTGTTG
AAAAAAGTGTGAAGAGGGACGTTAAA

Last Updated on 06.12.2018 16:11