Sequences from confirmation amplicon


Confirmation sequences for line 283E12 and hit At5g14740 are shown.
Line ID 283E12
Locus At5g14740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4761598
Primers used for PCR AU15 - 8474, show primer details
   
Sequence Name 51-K106290-0022-283-E12C-8409
GenBank Accession LN507511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106290-0022-283-E12C-8409
GAGCCATTTACATTGAATATATAAACTAACATGCATCCTATGAGTCAAGCAAGTTTTAGG
AAAAAACACAAAACAATCTAAAGAGAGAAGAATGTATATATTGATTATTGAGGGAGAGAT
TTTGACCTTAAGGTGCAAGACAGCGTATTCAATGGCGGCTCCAACTCCTGCATATTTGAC
CTGTAATTATTTTTATAAAGGAAAAAAAAAACAGAAAAGCCATGTTATGACTTCATAAGT
CAAAATCAAAGTCAAACACTCTGTTTGAGTCAAAATGTTCAAAAATAAATTAGCGATGTA
AATTAATGTAGTGTATAAAGTGTTTACCTTGTCAAAAGGAGGAACCATATTGGCGATATT
ACGAACCACGAAGGCTTCTCCAGGATGGAAGTCTAGTACGTGTGATGGGCACACTCGTGA
GTCCAAACAAGCAAACACCATGTACTGCACAATTCATAGTATATGACCATATAATATGAG
TCCAAAAGAAAAATCTAACATATTCATATAGAGATAACTATATTTTTCATCAACTGCTTT
ATATATTTTTCTTCTTATTTTTGTTTT
   
Sequence Name 51-K106291-0022-283-E12C-AU15
GenBank Accession LN507512 [GenBank]
Sequencing primer gene specific primer, AU15: 5'-AAAGCCCAAAGGTAAAACAAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106291-0022-283-E12C-AU15
TGAAAATTAGTTATCTCTATATGAATATGTTAGATTTTTCTTTTGGACTCATATTATATG
GTCATATACTATGAATTGTGCAGTACATGGTGTTTGCTTGTTCGGACTCACGAGTGTGCC
CATCACACGTACTAGACTTCCATCCTGGAGATGCCTTCGTGGTTCGTAATATCGCCAATA
TGGTTCCTCCTTTTGACAAGGTAAACACTTTATACACTACATTAATTTACATCGCTAATT
TATTCTTGAACATTCTGACTCAAACAGAGTGTTTGACTTTGATTTTGACTTATGAAGTCA
TAACATGGCTTTTCTGTTTTTTTTTTCCTTTATAAAAATAATTACAGGTCAAATATGCAG
GAGTTGGAGCCGCCTTTGAATACCCTGTCTTGCACCTTAAGGTCAAAATCTCTCCCTCAA
TAATCAATATATACATTCTTCTCTCTTTAGATGGTTTTGTGTTTTTTCCTAAAACTTGCT
TGACTCAAAGGAGGCAGGTTAGTTTATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAAATGTCC

Last Updated on 06.12.2018 16:11