SimpleSearch - Line and FST details


Line specific information

 
Line ID 283E12
Vector Used pAC161
Line Availability available as T3 set from NASC (N427132)
Segregation Analysis 50:50:49
Confirmed for Hit At4g36950 At5g14740
Parent of DUPLO pair none
Parent of pair(s) 95729, 95730

Gene hit At4g36950

 
Sequence (A. th genome BLAST matches underlined) 
>93-K015203-022-283-E12-8409
TTGATCCATGTAGAATTTCCCGGACATGAAGCACTTTACAATTGAATATATTCTCTTTTC
ACGTGTCTTATGACCCCTCTCACTATTCCGACCAATCACGTACCTTCTAACTCACCAAAC
ATGTAATCCTTTACTCCGGCGAGGCCTACCGATGAACCTTACAGTGACCGACTCATTATC
CGGAGATTGAACCGACTTCCGACCGGGAGACCTGAATGGATTCCTCTGTGAGACCGAACA
AGCTTCGTCTTCTTCGCCACTCTCATCATCAACGGCTAAGAATGGATGATCCGAAAGCAT
CTATGGCGTCCATCTCTCCGCGGCGTTTTTAACAAAACACTTGCTCACGAAATCTTTACC
TTCCTCCGACGATCCACCGGAATACTTGGAACTTCATCGCCGGAACCAATACGAACCAAT
AAAGACGTAACATTTTTATTAGCACCATCCTCCAAACACCATGCCGTTTTACCACTATGG
GATCCT
GenBank Accession AL944631 [GenBank]
Graphic View Graphic view of gene At4g36950
Predicted Position of Insertion Chr4:17422834 - go to primer design
BLAST e Value 1e-127
Hit Clone Code (BAC ID) AP22
Hit Gene Code At4g36950 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation mitogen-activated protein kinase kinase kinase 21
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details

Gene hit At5g14740

 
Sequence (A. th genome BLAST matches underlined) 
>93-K015178-022-283-E12-8409
CTGATCCATGGTAGATTTCCCGGACATGAANGCCTTTACAATTGAATATATTGCACTTAT
CTGTCTTTCTTTTAGTCCACCATACTTGTGTGACCGGCACCCAACGTTCTGCTTAGAGAA
CACACTGTGTACTGTGATCATTGACCGGATAGACGGTTACCGGATCGCGCTGGACGTGCT
ATCCACAGGAGGGACACCCTTCCGACCTTTTGACCCGGCTATATGATTCTCCCTGAAGAG
AGAACCCGAAAAGCCTTGTTATGACTTCATAATTCAAAATCAAAGTCAAACCCTCTGTTT
GAGTCAAAATGTTCAAAAATAAATTGGCGATGTAAATTAATGTAGGGTATAAAGGGTTTA
CCTTCGTAAAAGGAGGAACCATATGGGCGATTTTACAAACCACCAAGGCATCCCCAGGAT
GGAAGTCTA
GenBank Accession AL944630 [GenBank]
Graphic View Graphic view of gene At5g14740
Predicted Position of Insertion Chr5:4761582 - go to primer design
BLAST e Value 3e-60
Hit Clone Code (BAC ID) T9L3
Hit Gene Code At5g14740 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation carbonic anhydrase 2
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37