Sequences from confirmation amplicon


Confirmation sequences for line 052H11 and hit At5g14870 are shown.
Line ID 052H11
Locus At5g14870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4810460
Primers used for PCR UK07 - 8474, show primer details
   
Sequence Name 07-K019548-022-052-H11C-UK07
GenBank Accession LN489057 [GenBank]
Sequencing primer gene specific primer, UK07: 5'-TCCTCTCCTTCTTCCATAATCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K019548-022-052-H11C-UK07
CAAAATGCATAAACCCAAAAAAACAAAAAATCACACGACGCAACCACCTAGCCTCTCTGT
CCATCAATCTCAATCTCTCTCTGTGTTAATGCGGAATCCGATTTAACACCGGAATCCGCC
ATGGAAGATGAATAAAATCCGGTCTCTCCGCTGCCTCCTCCCGGAGACCATAACCTCCGC
CTCCACCGCCGCCTCAAATCGAGGATCTGACGGTAGCCAATTCAGCGTCCTATGGCGGCA
TCAGATCCTGGACCCGGACAGCAACATCGTCACCTATTGGAACCACGTCTTTCTCATTAC
CTCAATCCTCGCTCTCTTCCTCGATCCTTTCTATTTCTACGTCCCTTACGTCGGTGGTCC
GGCGTGTCTCTCCATCGACATCAGCCTCGCCGCTACTGTCACTTTCTTCCGTACAGTCGC
CGATATCTTCCACCTCTTGCACATTTTCATGAAATTCAGAACTGCGTTCGTCGCGAGGAG
TTCTCGTGTTTTTGGTCGCGGTGAGCTTGTTATGGATTCTAGAGAGATTGCTATGAGATA
CTTGAAGACAGATTTCCTCATTGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCANCAATGAGTATGATGGTCAATATNGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGA
   
Sequence Name 08-K019548-022-052-H11C-8409
GenBank Accession LN489524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K019548-022-052-H11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATCAATGAGGAAATCTGTCTTCAA
GTATCTCATAGCAATCTCTCTAGAATCCATAACAAGCTCACCGCGACCAAAAACACGAGA
ACTCCTCGCGACGAACGCAGTTCTGAATTTCATGAAAATGTGCAAGAGGTGGAAGATATC
GGCGACTGTACGGAAGAAAGTGACAGTAGCGGCGAGGCTGATGTCGATGGAGAGACACGC
CGGACCACCGACGTAAGGGACGTAGAAATAGAAAGGATCGAGGAAGAGAGCGAGGATTGA
GGTAATGAGAAAGACGTGGTTCCAATAGGTGACGATGTTGCTGTCCGGGTCCAGGATCTG
ATGCCGCCATAGGACGCTGAATTGGCTACCGTCAGATCCTCGATTTGAGGCGGCGGTGGA
GGCGGAGGTTATGGTCTCCGGGAGGAGGCAGCGGAGAGACCGGATTTTATTCATCTTCCA
TGGCGGATTCCGGTGTTAAATCGGATTCCGCATTAACACAGAGAGAGATTGAGATTGATG
GACAGAGAGGCTAGGTGGTTGCGTCGTGTGATTTTTTGTTTTTTTGGGTTTTTGCATTTT
GTTTTTGATGATTATGGA

Last Updated on 06.12.2018 16:11