Sequences from confirmation amplicon


Confirmation sequences for line 102C06 and hit At5g15470 are shown.
Line ID 102C06
Locus At5g15470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5023496
Primers used for PCR 03kn - 8474, show primer details
   
Sequence Name 73-K113669-0022-102-C06C-03kn
GenBank Accession LN512939 [GenBank]
Sequencing primer gene specific primer, 03kn: 5'-CTGTTGCCTAATTCAAGGATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113669-0022-102-C06C-03kn
TTCAGTTTCTTCTTAGTGTTGTTCCTTGGCAAAACATCTTGTTAGTTTTTTGTTGTATCC
ATTTGTGATGCTTAGGATTGTACTTGGATTGGCTTAAGTCAACAACGTTTGGTTGATTTT
GGATTCTTCTTGTTAGGTCCCCACTAATGATTTTGTAAGATCCTGGTTTTCATTGAGCTG
TACTACCTTAATTGTTAGGTGTACATATACTTTTCACCATCTATTTCTCACTTTTCCATA
TCAATGCATTTTGTTGGATTGTGGAGCTGAATGACAAATTTTGTTGAATTCCTTTTTTTT
TTGGGTGTTAAAATTTCTCTGCATTACTAAATTTGAACCAAATTATATTTCTTTAGGGTC
TTCAATTGTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCATTGATT
ATAATGGTCAATATGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTTCAAAAAT
GTAAATTTCCC
   
Sequence Name 42-K113666-0022-102-C06C-8409
GenBank Accession LN504533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113666-0022-102-C06C-8409
TTTACATTGAAGACCCTAAAGACATATAATTTGGTTCAAATCTAGTAATGCAGAGAAATC
TTAACACACAAAAAAAAAAGGATTTCACCAAATTTTGTCTTTCACCTCCACAATCCAACA
AAATGCATTGATTTGGAAAAGTGAAAAATAAATGGTGAAAAGTATTTGTCCCCCTAACAT
TTAAGGTAGTACAGCTCATTGAAAACCAGGATCTTACAAAATCTTTAGGGGGGACCTAAC
AAGAAAAATCCAAAATCACCCAAACTTTGTTGACTTAACCCAATCCAAGTACAATCCTAA
CCTTCACAAATGGATCCACCAAAAAACTAACAGGATGTTTTTGCCAGGGAACACCCCTAA
AAAAAAAAACTTGTAAAACTTTTCTTTCCCCAACAAAAAACCCTTGATTAGGGCAAC

Last Updated on 06.12.2018 16:11