Sequences from confirmation amplicon


Confirmation sequences for line 304G08 and hit At5g17740 are shown.
Line ID 304G08
Locus At5g17740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5857846
Primers used for PCR 18xc - 8474, show primer details
   
Sequence Name 33-K119444-0022-304-G08C-18xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18xc: 5'-GGGTATATAGGAGTTCACGACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K119444-0022-304-G08C-18xc
TCAAGATTCTCGTTGAATGTTTCCCTGATAGCTTTGTCCATACTCTTCAACCATAGTATT
ATTCTTGTTTCTAGCAAGAAACCTCCACTTGAGCTTAATGCCATTGTAGACATCGGAGAC
AACCTCTCCATCGCTAAGGTATAACTTGACTTTCTTCTCCGCAGGGTCTCTAGCCATAAT
TAGCCTGACAGAATTTGGACTTATCTTGGTGGAGAGATAAGCCTGAGCAGCTGCGTAAAG
CTCGTCGGGAATATACATCGAGGACATTTGATCGATGGTAAGCGTGAGAGTCGATGAACG
AGAGCCAGCGAAGGACTTGAGATAAGAGAAAACGAAGTTTTGAACTGGACGTGGAATAAT
TGTGTTAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATATCCGCAACGTTATT
   
Sequence Name 01-K119443-0022-304-G08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119443-0022-304-G08C-8409
CATTTACATTGATTACACAATTATTCCACGTCCAGTTCAAACTTCGTTTTCTCTTATCTC
AAGTCCTTCGCTGGCTCTCGTTCATCGACTCTCACGCTTACCATCGATCAAATGTCCTCG
ATGTATATTCCCGACGAGCTTTACGCAGCTGCTCAGGCTTATCTCTCCACCAAGATAAGT
CCAAATTCTGTCAGGCTAATTATGGCTAGAGACCCTGCGGAGAAGAAAGTCAAGTTATAC
CTTAGCGATGGAGAGGTTGTCTCCGATGTCTACAATGGCATTAAGCTCAAGTGGAGGTTT
CTTGCTAGAAACAAGAATAATACTATGGTTGAAGAGTATGGACAAAGCTATCAGGGAAAC
ATTCAACGAGAATCTTTGGAGCTGAGCTTCGACAAGAAACACAGAGACTTGGTCGTGAAC
TCCTATATACCCACTTCCTTCACAATTTCACAAGAGTCATATTCAAAGCGTCTGACAT

Last Updated on 06.12.2018 16:11