Sequences from confirmation amplicon


Confirmation sequences for line 347F07 and hit At5g17930 are shown.
Line ID 347F07
Locus At5g17930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5943472
Primers used for PCR JU24 - 8474, show primer details
   
Sequence Name 35-K021167-022-347-F07C-8409
GenBank Accession LN501655 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K021167-022-347-F07C-8409
GATGGATCGAATGATTTATTCGAAGGACTTCCATCTGTACTTGATTCGATGGGATCTGAA
CTCGGTGATTCCCGAAAGAAACGTAAGAAAAAGAGATCAGAAGAAAAACAGGACCATGAG
GATGTCGATGAGCTAGCAAATGAAGATTTGGAGCATGAAGAATCTGAATTTTCTGACGAG
GAAAGCGAAGAAGAGCCTGTAGGAAAAAGAGACCGTAA
   
Sequence Name 39-K021167-022-347-F07C-JU24
GenBank Accession LN503205 [GenBank]
Sequencing primer gene specific primer, JU24: 5'-TATGCCTTTTACGGTCTCTTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K021167-022-347-F07C-JU24
TCTTCTTCGCTTTCCTCGTCAGAAAATTCAGATTCTTCATGCTCCAAATCTTCATTTGCT
AGCTCATCGACATCCTCATGGTCCTGTTTTTCTTCTGATCTCTTTTTCTTACGTTTCTTT
CGGGAATCACCGAGTTCAGATCCCATCGAATCAAGTACAGATGGAAGTCCTTCGAACAAA
TCATTCAATCCATCATCCATGCCCCTTAGTTTTCCTTTCTTTATCTTGAGCTTCTTTGCG
AGCCTCCTCTCAAGTTCAGCATCCTGGTCTCCAGATATTACAGTTGGTGTATCCATTTCA
AGAAACTCTTCAAAATTCGTCTTCTTCCTTGGTCTATTCAGATCCTTTATTCTCTCTATT
CAGATCCTTTATTCAGATCCTTCAAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11