Sequences from confirmation amplicon


Confirmation sequences for line 823C07 and hit At5g18920 are shown.
Line ID 823C07
Locus At5g18920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6311075
Primers used for PCR 11ne - 8474, show primer details
   
Sequence Name 31-K114626-0022-823-C07C-11ne
GenBank Accession LN500271 [GenBank]
Sequencing primer gene specific primer, 11ne: 5'-AAAGATGTTCAAGCTTTGAAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114626-0022-823-C07C-11ne
AGATTAGAAAAGCCATACTTGATTAGTTACTGCTTATAGAGGAACCATATAAGTATGACA
TTTTTGTCGTCTTGCAAAATTGATTAATAAAACGGTGTCTTGTGATTGTGTTCTTGTTGG
GGGGATTGCAAAATTTACAAGCTTTCATTCAATGTAATCTTGGTTTTATTTTGTTGCTAT
TTGATTTTGGTTCATTGCATAGAATCTTTCTCTTTACTCTTAAGACTTCTCTTTCTGACA
CTTATTTCGGATAAGACAATAGTGATGGTGTTTTGAGACAGTTTGATTTGTTTTCTTCGT
GGTAGAATTTGGCTCTGCTTTATAATGAGATCTTGAACGTTTGACTCCTTTGCGAAGAAC
CTTTGTACATCACTCTTCCAGAAACTATATAAGAAGAGCAAAACAAGCTCGTTAAAAGAT
TTGATTTTTGATTTATTTACTTGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 31-K114625-0022-823-C07C-oriL
GenBank Accession LN500270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114625-0022-823-C07C-oriL
TGAATAATCCCAGTAAATAAATCAAAAATCAAATCTTTTAACGAGCTTGTTTTGCTCTTC
TTATATAGTTTCTGGAAGAGTGATGTACAAAGGTTCTTCGCAAAGGAGTCAAACGTTCAA
GATCTCATTATAAAGCAGAGCCAAATTCTACCACGAAGAAAACAAATCAAACTGTCTCAA
AACACCATCACTATTGTCTTATCCGAAATAAGTGTCAGAAAGAGAAGTCTTAAGAGTAAA
GAGAAAGATTCTATGCAATGAACCAAAATCAAATAGCAACAAAATAAAACCAAGATTACA
TTGAATGAAAGCTTGTAAATTTTGCAATCCCCCCAACAAGAACACAATCACAAGACACCG
TTTTATTAATCAATTTTGCAAGACGACAAAAATGTCATACTTATATGGTTCCTCTATAAG
CAGTAACTAATCAAGTATTGGCTTTTCTTAATCTCTATCATTTCTTCATTCTCCTTTCAT
GACATTCCTTCAAAGCTGAAACATCT

Last Updated on 06.12.2018 16:11