Sequences from confirmation amplicon


Confirmation sequences for line 015D05 and hit At5g19120 are shown.
Line ID 015D05
Locus At5g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6415731
Primers used for PCR MP39 - 3144, show primer details
   
Sequence Name 49-K023831-022-015-D05C-3144
GenBank Accession LN506701 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K023831-022-015-D05C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTTTAATCAGAGCGTGTTCTTTGTCCAAAGCCCATCATTGAGTTACCCAAA
TCAAAGTCCAATATAAAGCCTTCTAATTGTAGCCCACCCATTACAATCGGGTTGACTCGA
CTTGACCCGCCGTCAACAATCCCCGAGCATCGGACTCCACCACCAACATCCACCATCAGA
TTCTTCCCATGAATCCTCCACCGCACCATCTCACTCTGCAACGCTAGATCTACCGCCGGA
AAATCTACGTCACTAGTGAAGCAGAGCCCAAACGGTGCGACAGGAGGCACCGATGTAGCT
TCACCCGCAGCTTTGGCGTAAGCTTCCGCGAACACCTTGTAGATC
   
Sequence Name 53-K023831-022-015-D05C-MP39
GenBank Accession LN508020 [GenBank]
Sequencing primer gene specific primer, MP39: 5'-AGAGAGCTCGATCTACAAGGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K023831-022-015-D05C-MP39
AGCTGCGGGTGAAGCTACATCGGTGCCTCCTGTCGCACCGTTTGGGCTCTGCTTCACTAG
TGACGTAGATTTTCCGGCGGTAGATCTAGCGTTGCAGAGTGAGATGGTGCGGTGGAGGAT
TCATGGGAAGAATCTGATGGTGGATGTTGGTGGTGGAGTCCGATGCTCGGGGATTGTTGA
CGGCGGGTCAAGTCGAGTCAACCCGATTGTAATGGGTGGGCTACAATTAGAAGGCTTTAT
ATTGGACTTTGATTTGGGTAACTCAATGATGGGCTTTGGACAAAGAACACGCTCTGATTA
AACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCC
TCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCCAAGGATAGTGGGATT
GTGCGTCCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAAACGTG
GTTGGAACCTCTTCTTTTTCCACCATGCT
Primers used for PCR FI05 - 8474, show primer details
   
Sequence Name 28-K028097-022-015-D05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K028097-022-015-D05C-8409
CTGCTGTGGTTTCAATCGTGGAATCCGAGTAAGCATAGGATGGTTGAGGTTACAGTGC
   
Sequence Name 28-K028097-022-015-D05C-FI05
GenBank Accession LN498703 [GenBank]
Sequencing primer gene specific primer, FI05: 5'-TCCATACAAGGAAGGTCACAGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K028097-022-015-D05C-FI05
TTGGAATTTGTCATATGTTCTAGTTGTCGCATGGTAATTAGCAGTTAGAGGTCTGATGTA
TCGTTAGAAAACTAAAAATGCGATGAATGAGTGAAGTTTCTGTTTTTTGTATTGATTTTT
AGTTTTAAGCACTGTATTTTTACTTCATTTAAGTAATAGCTGTTTCTTAAATTAGATCTA
TTAGTTGTACAAATGCATGATTGCTTGTAGCAGGTATTGTAAGAAAAGAGTACACTCAAA
AGAGATCAAAATTGAGGATATCTACCAGATAATTAGAAGGTTACAATTACAAAGGTTTCT
GTATTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11