Sequences from confirmation amplicon


Confirmation sequences for line 120C08 and hit At5g21030 are shown.
Line ID 120C08
Locus At5g21030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7140645
Primers used for PCR KD04 - 8474, show primer details
   
Sequence Name 29-K017121-022-120-C08C-KD04
GenBank Accession LN499075 [GenBank]
Sequencing primer gene specific primer, KD04: 5'-AAAGAATTACCAAGAGAGATGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K017121-022-120-C08C-KD04
GCTCCGCTGGTAGCTACAAGTAATAAAGCAAAGAATTAAGAGATTAATGGCTAAATTCAA
TACAGAGATTTAATGTATCAGGTAAAAAAAAGTTTGAAGTGATGTGCTGCTTACACATAG
GATAGTGAATGCACAAGTTCTTGGAGTTGATCTGTGTCAAATCCAATCTCGTCGTAGAGC
ACATGGTAATGTGTTGGCCTTGTAGTTCCCTAACACATCGAAAAGTGTTTTTAAGTTTTT
GTTCGATTCATAAAGGATTCGAGCATAATAATATATATACTTATCACTTACAATCTTTCC
AGCATGAGCACAAAGATAGAAATCGTTGTTGTGTTGGTGACAGATGTTGCTGTCAATTAT
TGTTCCTGCGGGTAAATAGAGAACAGGTTTCAAGTCATTTCAACAACACTAACAAACAAA
CAAAACACAAAAAAATATCTGCAACTGTTTACCTGGAAGAACATTATTAGGGCTTTCGGT
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATGGGCGAAAGCAATAAA
   
Sequence Name 30-K017121-022-120-C08C-8409
GenBank Accession LN499512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K017121-022-120-C08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAACCGAAAGCCCTAATAATGTTCTTCCAGGT
AAACAGTTGCAGATATTTTTTTGTGTTTTGTTTGTTTGTTAGTGTTGTTGAAATGACTTG
AAACCTGTTCTCTATTTACCCGCAGGAACAATAATTGACAGCAACATCTGTCACCAACAC
AACAACGATTTCTATCTTTGTGCTCATGCTGGAAAGATTGTAAGTGATAAGTATATATAT
TATTATGCTCGAATCCTTTATGAATCGAACAAAAACTTAAAAACACTTTTCGATGTGTTA
GGGAACTACAAGGCCAACACATTACCATGTGCTCTACGACGAGATTGGATTTGACACAGA
TCAACTCCAAGAACTTGTGCATTCACTATCCTATGTGTAAGCAGCACATCACTTCAAACT
TTTTTTTACCTGATACATTAAATCTCTGTATTGAATTTAGCCATTAATCTCTTAATTCTT
TGCTTTATTACTTGTAGCTACCAGCGGAGCACAACTGCCATCTCTCTTG

Last Updated on 06.12.2018 16:11