Sequences from confirmation amplicon


Confirmation sequences for line 428D09 and hit At5g21482 are shown.
Line ID 428D09
Locus At5g21482 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7229548
Primers used for PCR 36hw - 8474, show primer details
   
Sequence Name 57-K112758-0022-428-D09C-36hw
GenBank Accession LN509468 [GenBank]
Sequencing primer gene specific primer, 36hw: 5'-GTTTTATGGCATGTGTTTATCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K112758-0022-428-D09C-36hw
AGGCTCCCAAGTCGCAAGTCGTGTGATCACAAATTGTCGCCAATGTGGCACACGTGTCTT
TCTTCAGTCCCCTCGTCATTTTTTTTACCCGTACGGGGTTTTAAGTACAATAAAAGTTGG
AATTTAGTGTGGTCGTTTAGATTTTGTAGGCGAGATAAAAAAAAGAATACTAAACTAATA
CGATGCCGTATTAGGTATTACGGTTGGGTGGGTGACGGATATGTATTGTAACCGTCGTTA
AGGTTAGCGTCATATAGGGAAAGAGATGAAATTTGTAGGGACCCAATATGAACTAACGTT
AAATTTTTTATTTTACATTTCTAAAATAGCCTTTTGTAGGTTACTGAAGGGTAGTTTCGT
CTTTATATGTTTTACTTTATGTAGATTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 17-K112757-0022-428-D09C-8409
GenBank Accession LN493922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112757-0022-428-D09C-8409
TTACATTGAATATATCCTGAAATCTACATAAAGTAAAACATATAAAGACGAAACTACCCT
TCAGTAACCTACAAAAGGCTATTTTAGAAATGTAAAATAAAAAATTTAACGTTAGTTCAT
ATTGGGTCCCTACAAATTTCATCTCTTTCCCTATATGACGCTAACCTTAACGACGGTTAC
AATACATATCCGTCACCCACCCAACCGTAATACCTAATACGGCATCGTATTAGTTTAGTA
TTCTTTTTTTTATCTCGCCTACAAAATCTAAACGACCACACTAAATTCCAACTTTTATTG
TACTTAAAACCCCGTACGGGTAAAAAAAATGACGAGGGGACTGAAGAAAGACACGTGTGC
CACATTGGCGACAATTTGTTGATCACACGACTTGCGACTTGTGGAGCCTTTTTTTAGTTT
AAGCATGCACGACTAATCATCACACGTGATAAACACATGCCATAAAA

Last Updated on 06.12.2018 16:11