Sequences from confirmation amplicon


Confirmation sequences for line 800C02 and hit At5g22060 are shown.
Line ID 800C02
Locus At5g22060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7305326
Primers used for PCR 50kh - 8474, show primer details
   
Sequence Name 41-K113531-0022-800-C02C-50kh
GenBank Accession LN504149 [GenBank]
Sequencing primer gene specific primer, 50kh: 5'-GAAGCAAAGTCTTCAAAACCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113531-0022-800-C02C-50kh
TTTATTTTTTTTAGGGTTTTCGTAAGAACAGAGCAAAAAACATGTTTGGAAGAGGACCTT
CAAGGAAGAGCGATAACACAAAGTTCTACGAGATCCTTGGTGTTCCTAAGACCGCAGCAC
CAGAAGATCTCAAGAAAGCTTATAAGAAAGCCGCTATCAAAAACCATCCTGATAAGGGTG
GTGATCCCGAAAAGGTTTCTCTTTTCGATTCTCGCTTAGATTCTCTGTAAATTCAATTCG
TTAATCTAGTAGTTTGATTTGAATTCTCCGATTGATATAGGCTTAGATCTTCGTATTAGT
ATTACGAATGTTCGATTCGATCTGAAATCTGCGAGTTTTAGGTCAACTATNGATGGTTGT
TTTTGTCTTGTGATAGTTAAAAGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K113530-0022-800-C02C-8409
GenBank Accession LN493506 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113530-0022-800-C02C-8409
CATTTCACTTTTACTATCACAAGACAAAACAACCATCAATAGTTGACCTAAAACTCGCAG
ATTTCAGATCGAATCGAACATTCGTAATACTAATACGAAGATCTAAGCCTATATCAATCG
GAGAATTCAAATCAAACTACTAGATTAACGAATTGAATTTACAGAGAATCTAAGCGAGAA
TCGAAAAGAGAAACCTTTTCGGGATCACCACCCTTATCAGGATGGTTTTTGATAGCGGCT
TTCTTATAAGCTTTCTTGAGATCTTCTGGTGCTGCGGTCTTAGGAACACCAAGGATCTCG
TAGAACTTTGTGTTATCGCTCTTCCTTGAAGGTCCTCTTCCAAACATGTTTTTTGCTCTG
TTCTTACGAAAACCCTAAAAAAAATAAACAGAGAGAAAAGTCTTCACGAGGAGAAGGAGA
AGGAGAATGTTGTGGCTGCTAACTTGTTTGGTTTTGAAAANTTTGCTT

Last Updated on 06.12.2018 16:11