Sequences from confirmation amplicon


Confirmation sequences for line 011A07 and hit At5g22830 are shown.
Line ID 011A07
Locus At5g22830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7629237
Primers used for PCR RK95 - 8474, show primer details
   
Sequence Name 39-K021972-022-011-A07C-8409
GenBank Accession LN503208 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K021972-022-011-A07C-8409
AGATTTCCCGGACATGTAGATTTCAGTGTCAGCTCTTTTATCCTATGCTCAGTTCAAACT
TAAACTTATAGCAAAAGGAAATGCTTTGTGTGTCGAAAATGGCAGTTTCATCCTCTATAG
CACGAATCCGTTCCTTTTAAGATCTAACTTAGTCATGGAGAAGTCCTAGTCTAGCAGTCA
AAGTGAAGAAGATCTAACTGAAATATACTCACTAGCTTTTCATACTCACACGGGGTTCAA
TGTCCATAAGCCTTTGCTCTAATCGTTGTATCCTTGAGATTAGCGCCGATTCAACAGCCT
TCAATAAAGCCAGAGTAATGAAAGGTTAACACCAAAATGATTGGAATTATAACAAACTCA
AGTTATGCAGGGCTGAAGAGATAATATTTATAATCTTTTATCTGTGTACAAAACATATAT
TTCTACATAAACAAATCATCATCCAATCAAATTATTTATGGGCCAAACCAACCTCTAGTT
CAAATGGCATAGAGGGTCCTCCATTCATA
   
Sequence Name 40-K021972-022-011-A07C-RK95
GenBank Accession LN503564 [GenBank]
Sequencing primer gene specific primer, RK95: 5'-TAACCCAAGAAGTATGAATGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K021972-022-011-A07C-RK95
CCTCTATGCCATTTGAACTAGAGGTTGGTTTGGCCCATAAATAATTTGATTGGATGATGA
TTTGTTTATGTAGAAATATATGTTTTGTACACAGATAAAAGATTATAAATATTATCTCTT
CAGCCCTGCATAACTTGAGTTTGTTATAATTCCAATCATTTTGGTGTTAACCTTTCATTA
CTCTGGCTTTATTGAAGGCTGTTGAATCGGCGCTAATCTCAAGGATACAACGATTAGAGC
AAAGGCTTATGGACATTGAACCCCGTGTGAGTATGAAAAGCTAGTGAGTATATTTCAGTT
AGATCTTCTTCACTTTGACTGCTAGACTAGGACTTCTCCATGACTAAGTTAGATCTTAAA
AGGAACGGATTCGTGCTATAGAGGATGAAACTGCCATTTTCGACACACAAAGCATTTCCT
TTTGCTATAAGTTTAAGTTTGAACTGAGCATAGGATAAAAGAGCTGACACTGAAATCTAC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11