Sequences from confirmation amplicon


Confirmation sequences for line 052G08 and hit At5g22910 are shown.
Line ID 052G08
Locus At5g22910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7663114
Primers used for PCR 64hw - 8474, show primer details
   
Sequence Name 66-K114067-0022-052-G08C-64hw
GenBank Accession LN511662 [GenBank]
Sequencing primer gene specific primer, 64hw: 5'-AGGACTAAGATGATCAATGCCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114067-0022-052-G08C-64hw
AGACCGTTTCAACTATTATAGCAAATAATGTTCGTTAGATAGTAGATATACATTACTAAT
CAAACACTCCGGTGCCGTCTATTGTGTAGATAACAATTAGTATTGCTTTATAGACAATAA
AACGGTAACTTTTCGTTGATCAAACTTTTAACCAGTTTTAGAGCATTATATATATTGCTT
TAGGTCTCCGGATTTGTAGACTTTGATTACTACAAAAGTATATTTAATGAGGTAAGTAGT
TTTGTATGCAAATGCACCAATTAGATTCCTAAATTAATCGACGTCTTACATACCTGTATG
TTCCAACGTAGGAGATGCATACTAGAGTGAGAAATCCAAGGAAATCGCTGACGGCGGCTG
TAGATATGGCGATGCGGCCGAGCTCGGAGTTTGACATCTTCAGCTCATCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 41-K114066-0022-052-G08C-8409
GenBank Accession LN504158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114066-0022-052-G08C-8409
CATTTACATTGAATATATCCTGATGAGCTGAAGATGTCAAACTCCGAGCTCGGCCGCATC
GCCATATCTACAGCCGCCGTCAGCGATTTCCTTGGATTTCTCACTCTAGTATGCATCTCC
TACGTTGGAACATACAGGTATGTAAGACGTCGATTAATTTAGGAATCTAATTGGTGCATT
TGCATACAAAACTACTTACCTCATTAAATATACTTTTGTAGTAATCAAAGTCTACAAATC
CGGAGACCTAAAGCAATATATATAATGCTCTAAAACTGGTTAAAAGTTTGATCAACGAAA
AGTTACCGTTTTATTGTCTATAAAGCAATACTAATTGTTATCTACACAATAGACGGCACC
GGAGTGTTTGATTAGTAATGTATATCTACTATCTAACGAACATTATTTGCTATAATAGTT
TGAAACGGTCTGCAGGTATGTATCGCCCGGAATAGCCNACCGCGACATAGTGGCATTGAT

Last Updated on 06.12.2018 16:11