Sequences from confirmation amplicon


Confirmation sequences for line 052G08 and hit At5g22940 are shown.
Line ID 052G08
Locus At5g22940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7677366
Primers used for PCR MK63 - 8474, show primer details
   
Sequence Name 81-K101329-0022-052-G08C-8409
GenBank Accession LN514041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K101329-0022-052-G08C-8409
CCATTTACATTATTGTATTTCTTCACATCTTACTTCTCCGTCGAAGACCAATCCTCACCG
TCGTCAATACGTCTCTTGTCTAACCACAAAACGTCGTCGTCTCTCCCATCTCGTGCTCTC
ATCGAATCTTCCGCCATTAAAACCACCTCCATTGGTAAAAAAGCCTCGATCTTTATCTCT
TCTCTGTTTCAAATCTTTCACTTCTCCTCAAAAATTGATTTTTTGGGGTCTCTGATTAAA
GGTTTGTTCACCGGAATGAAGATATACGTTTACGATTTACCGGCGAGTTATAACGACGAT
TGGGTTACAGCATCAGACCGTTGCGCTAGCCACCTCTTCGCGGCGGAGGTAGCTATCCAC
CGTGCACTTCTCTCCTCCGACGTCCGTACGTTAGACCCAGATGAAGCTGATTACTTCTTC
GTCCCTGTCTACGTTTCTTGCAATTTCTCCACATCCAACGGCTTCCCTTCTCTAAGCCAC
GCTCGCTCTCTCCTTAGCTCCGCCGTCGATTTTCTCTCCGATCATTACCCATTTTGGAAC
AGGAGTCAAGGCTCTGACCACGTCTTCGTCGCTTCCCATGACTTTGGCGCAAGCTTCCAC
GCAATGGTNAGCTCCAAAGT
   
Sequence Name 81-K101335-0022-052-G08C-MK63
GenBank Accession LN514042 [GenBank]
Sequencing primer gene specific primer, MK63: 5'-TTAAACATGAGGACTTTGGAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K101335-0022-052-G08C-MK63
GCAAGTCTGGGAGCGACGAGACGTGGTCAGAGCCTTGACTCCTGTTCCCATTGGGTAATG
ATCGGAGAGAAAATCGACGGCGGAGCTAATGAGAGAGCGAGCGTGGCTTAGAGAAGGGAA
GCCGTTGGATGTGGAGAAATTGCAAGAAACGTAGACAGGGACGAAGAAGTAATCAGCTTC
ATCTGGGTCTAACGTACGGACGTCGGAGGAGAGAAGTGCACGGTGGATAGCTACCTCCGC
CGCGAAGAGGTGGCTAGCGCAACGGTCTGATGCTGTAACCCAATCGTCGTTATAACTCGC
CGGTAAATCGTAAACGTATATCTTCATTCCGGTGAACAAACCTTTAATCAGAGACCCCAA
AAAATCAATTTTTGAGGAGAAGTGAAAGATTTGAAACAGAGAAGAGATAAAGATCGAGGC
TTTTTTACCAATGGAGGTGGTTTTAATGGCGGAAGATTCGATGAGAGCACGAGATGGGAG
AGACGACGACGTTTTGTGGTTAGACAAGAGACGTATTGACG

Last Updated on 06.12.2018 16:11