Sequences from confirmation amplicon


Confirmation sequences for line 573H03 and hit At5g23000 are shown.
Line ID 573H03
Locus At5g23000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7696518
Primers used for PCR 41iv - 8474, show primer details
   
Sequence Name 65-K113020-0022-573-H03C-41iv
GenBank Accession LN511435 [GenBank]
Sequencing primer gene specific primer, 41iv: 5'-TCTTCCTTAGCTTTGTGTTCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113020-0022-573-H03C-41iv
ATGAGCTGCTATTATTGACCACCTACAGTTTCAATTGATGTATATTAATAGCTAATATTG
GAGAAAAGTATAGCACTCTTGATGAATTAAAGTTGCTCGTAGTTTTGACTAAATCATGGA
ATCAATGTAGCTCGTGATTTTGACTAGATTTATGAAGAAATTAAGCACGTTTGGAAATAT
TTTGATCATTACTTCAAAATAACACATAAATTCAAAATTAGATTTAGGAGAAATTTTGTT
ACTATTATAAGATTAATTGAAGGAGAGTGTGAATTAATATTTTCTTTAAACCTATCTAAG
CAGATAACTATGAAAGGGCCTTCACAGGCAAGTGGGCGAAAAAATGATTTCATTTCTACA
TTTTTTGTAAGGATCTTATATGATTAAAGATGATTTCAAAAGGAATTAATTAGAGAACCT
GCTTCCTATGGCAGCGAAGAGACTAAAAATGATCCTGTCTTCTTCCTCAGAGAAGTCACC
ATGCTTTATGTTTGGACACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 33-K113019-0022-573-H03C-8409
GenBank Accession LN501068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113019-0022-573-H03C-8409
GCATTTCATTGAATATATCCTGTGTCCAACATAAAGCATGGTGACTTCTCTGAGGAAGAA
GACAGGATCATTTTTAGTCTCTTCGCTGCCATAGGAAGCAGGTTCTCTAATTAATTCCTT
TTGAAATCATCTTTAATCATATAAGATCCTTACAAAAAATGTAGAAATGAAATCATTTTT
TCGCCCACTTGCCTGTGAAGGCCCTTTCATAGTTATCTGCTTAGATAGGTTTAAAGAAAA
TATTAATTCACACTCTCCTTCAATTAATCTTATAATAGTAACAAAATTTCTCCTAAATCT
AATTTTGAATTTATGTGTTATTTTGAAGTAATGATCAAAATATTTCCAAACGTGCTTAAT
TTCTTCATAAATCTAGTCAAAATCACGAGCTACATTGATTCCATGATTTAGTCAAAACTA
CGAGCAACTTTAATTCATCAAGAGTGCTATACTTTTCTCCAATATTAGCTATTAATATAC
ATCAATTGAAACTTGTAGGTGGTCAATAATAGCAGCTCATCTACCGGGACGAACAGACAA
CGACATAAAAAACTATTGGAACACAA

Last Updated on 06.12.2018 16:11