Sequences from confirmation amplicon


Confirmation sequences for line 114C06 and hit At5g23020 are shown.
Line ID 114C06
Locus At5g23020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7719923
Primers used for PCR NB03 - 3144, show primer details
   
Sequence Name 20-K101268-0022-114-C06C-3144
GenBank Accession LN495143 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K101268-0022-114-C06C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATACGACGGA
TCGTAATTTGTCGTTTTATCAAAATGTACTTTCATTTTATAATAACGCTGCGGACATCTA
CATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTGACCATCA
TACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACATATCATTGTTTT
ATATTTATAATCTTGTCACCTCGATAATTTAAATTTGTTCGGAATTTTTGTTGATTAATC
TAACTCAGACAAAGGGAGAGCAATTACGTACGGATATTGTGTTGGCGGTAGCAACACCAA
GGTCGTTATGACAATGAATGGCGAAGACAATATCATCAGCCCCTGGAGTGTTTTCTATGA
CATAGGCCACGAGTTCTCCGAATTCTTGCGGCATGTTGATCCCGACCGTGTCCGCAAACC
CCACCGTGGTTGCACCCGCTTTTATCGATTCTCCTAGAATCTTACATATNANATCTTTCT
CCGTCCTGAATCACAAACTAGTATACTATAANATCTATATNTGACATGATCAAAACTGGG
CCAGATNACTGTATNAGGCACACCCTTATATNTNTCATTCTCATACCTNATATNANAACA
ACTTTCANAAAACAAAATTTTAAATTTCACAAAACAAATCA
   
Sequence Name 20-K101274-0022-114-C06C-NB03
GenBank Accession LN495144 [GenBank]
Sequencing primer gene specific primer, NB03: 5'-ACGCTTTTAGCTGATTTGTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K101274-0022-114-C06C-NB03
GTTTTTGAGTTGTTTTATATAGGTATGAGAATGAAAATATAAGGGTGTGCCTTATACAGT
TATCTGGCCCAGTTTTGATCATGTCATATATAGATTTTATAGTATACTAGTTTGTGATTC
AGGACGGAGAAAGATTTTATATGTAAGATTCTAGGAGAATCGATAAAAGCGGGTGCAACC
ACGGTGGGGTTTGCGGACACGGTCGGGATCAACATGCCGCAAGAATTCGGAGAACTCGTG
GCCTATGTCATAGAAAACACTCCAGGGGCTGATGATATTGTCTTCGCCATTCATTGTCAT
AACGACCTTGGTGTTGCTACCGCCAACACAATATCCGTACGTAATTGCTCTCCCTTTGTC
TGAGTTAGATTAATCAACAAAAATTCCGAACAAATTTAAATTATCGAGGTGACAAGATTA
TAAATATAAAACAATGATATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATCANACACTGATNGTTTAAACCGAAGGCGGGAAACGACAATCTG
ATCCCCGGGTACCCTGTCCTCTCCNAATGAAATGAACTTCCTTATATAGAGGAAGGGTCT
TGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGA

Last Updated on 06.12.2018 16:11