Sequences from confirmation amplicon


Confirmation sequences for line 454D05 and hit At5g23220 are shown.
Line ID 454D05
Locus At5g23220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7820107
Primers used for PCR 55rn - 8474, show primer details
   
Sequence Name 32-K116083-0022-454-D05C-55rn
GenBank Accession LN500719 [GenBank]
Sequencing primer gene specific primer, 55rn: 5'-TGGCCAGTTTCTTAAAATATGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116083-0022-454-D05C-55rn
ATACATATTGACCATCTACTTCCGCGCGCCTAACAATTTTTTAATATTGGAAGTAGTGGT
CAGTTTTATGTATGCAAGTACTGCTAATAATCACTTACTATATACAAGGGGTACTATTGT
TACTTTTATTTTCTTCGTCAACTGACTTTTATTAAAAAAAGACTATGAAAGGATACATGT
TGAGATTATGAACGCCTGCAGAAAAACAAAATAGCCAAAAGAGCGAAGCAACGCCTAAGA
GAAGGGAATCACATGAAAGATATTAGACAAAATACTTAAGAAAAAAAGCTCAATATGGTA
AGCATAGAGACTCCAACTTTGCTTCTCCAAATAACATGTAACGTCGGATAGCTTTTCCGC
GACGGTTGTGCGTGTAATTATCATTTTTTAGTTACCGAGTAGACTTCGCCGGAGTTTATC
GCAATCGACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 16-K116082-0022-454-D05C-8409
GenBank Accession LN493575 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116082-0022-454-D05C-8409
CGATTGCGATAAACTCCGGCGAAGTCTACTCGGTAACTAAAAAATGATAATTACACGCAC
AACCGTCGCGGAAAAGCTATCCGACGTTACATGTTATTTGGAGAAGCAAAGTTGGAGTCT
CTATGCTTACCATATTGAGCTTTTTTTCTTAAGTATTTTGTCTAATATCTTTCATGTGAT
TCCCTTCTCTTAGGCGTTGCTTCGCTCTTTTGGCTATTTTGTTTTTCTGCAGGCGTTCAT
AATCTCAACATGTATCCTTTCATAGTCTTTTTTTAATAAAAGTCAGTTGACGAAGAAAAT
AAAAGTAACAATAGTACCCCTTGTATATAGTAAGTGATTATTAGCAGTACTTGCATACAT
AAAACTGACCACTACTTCCAATATTTAAAAAATTGTTAGGCGCGCGGTAAGTAGATGGTC
AATAAAGTATTGTGATAGCTTTATTTTGTTGTCTTTATCCATATTTTAAAAAACTGGCCA
A

Last Updated on 06.12.2018 16:11