Sequences from confirmation amplicon


Confirmation sequences for line 340E05 and hit At5g25380 are shown.
Line ID 340E05
Locus At5g25380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8816319
Primers used for PCR BA32 - 8474, show primer details
   
Sequence Name 31-K104830-0022-340-E05C-BA32
GenBank Accession LN500154 [GenBank]
Sequencing primer gene specific primer, BA32: 5'-GTCCAAGATCCTCAGTTTTGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104830-0022-340-E05C-BA32
CATAATGTTGCAGAGGTTTGCATTACATAATCTCAATGCTTGGTCTTATTCTATCTTCCC
AAAAGATGATTTACATTTTGAGATTTTGAGTTACTTATATTAACCAATACTTTTTGTACA
GCTTGAACAAAGACCTTCAACTAGCTATATGGTGCAAGTGCAGCGAGATATCGATCCAAC
GATGCGCGGAATTCTGATTGATTGGCTCGTGGAGGTAGGAATACAATATTTGCTATGCAT
AGTTTTACACTTTTACTTGAACTTGTGTAAAAGTTGAAAGTGTGTTATTTTCAAAGTATT
TGCTAAACTAAGAATCTCTTTTCTTTCAGGTCTCTGAAGAGTACAAGTTGGTTTCAGTGG
ACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGNTTATT
   
Sequence Name 46-K104831-0022-340-E05C-8409
GenBank Accession LN505859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104831-0022-340-E05C-8409
GCCATTTACAATTGTCACTGAAACCAACTTGTACTCTTCAGAGACCTGAAAGAAAAGAGA
TTCTTAGTTTAGCAAATACTTTGAAAATAACACACTTTCAACTTTTACACAAGTTCAAGT
AAAAGTGTAAAACTATGCATAGCAAATATTGTATTCCTACCTCCACGAGCCAATCAATCA
GAATTCCGCGCATCGTTGGATCGATATCTCGCTGCACTTGCACCATATAGCTAGTTGAAG
GTCTTTGTTCAAGCTGTACAAAAAGTATTGGTTAATATAAGTAACTCAAAATCTCAAAAT
GTAAATCATCTTTTGGGAAGATAGAATAAGACCAAGCATTGAGATTATGTAATGCAAACC
TCTGCAACATTAATGCTGTCATATATACTTGCAGCATATAAGCTACAAAACTGAGGATTC
TTGGA
Primers used for PCR BA33 - 8474, show primer details
   
Sequence Name 46-K118809-0022-340-E05C-BA33
GenBank Accession LN506017 [GenBank]
Sequencing primer gene specific primer, BA33: 5'-ATGATTACCTGAGAAACGTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118809-0022-340-E05C-BA33
AAACTTTATCTCCATACTTAGTACCTGAAAAAATAGTGTATTGTATTTTTTGTTAATCTT
TTATTAAGAATGAATCATCTTAGCTGAGAACTGATAGAGATGGAGTCTGCATGTTAATTA
CTTCTAGTCTTGTGTATGTATTGTCTGTAATGAAGCAAAACTCCTCCAACCGCGGTGCAC
TAATCTCTTCGTATTTGCTTCAACAAGGAGGAATACCTTAGTTACTTGAGAAGTACTAAT
CTGTAATTATTAATTTAAGTATATATTCCAGAGTAAAACTTGCTCTAAGTATGCAATGAG
AGCAAACATGTGTTCCTCTGTGTCCACTGATTGTTCAGTTTTACTTTCATTGCATTGTGA
ATGAGGAAACAGTAAAAGTGGTAACTCACGAGGCTATTAACATGCAAGTGATACCAAGTA
GCTGGAGCTTCTGCTTTTCAATGTAATTATGAGACATGAACCGATCAATTAGCCTACATG
GATCAATTGATCAATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 14-K118808-0022-340-E05C-8409
GenBank Accession LN492734 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118808-0022-340-E05C-8409
TCGGACGAGCCATTTACATTGAATATATCCTGCAATTGATCAATTGATCCATGTAGGCTA
ATTGATCGGTTCATGTCTCATAATTACATTGAAAAGCAGAAGCTCCAGCTACTTGGTATC
ACTTGCATGTTAATAGCCTCGTGAGTTACCACTTTTACTGTTTCCTCATTCACAATGCAA
TGAAAGTAAAACTGAACAATCAGTGGACACAGAGGAACACATGTTTGCTCTCATTGCATA
CTTAGAGCAAGTTTTACTCTGGAATATATACTTAAATTAATAATTACAGATTAGTACTTC
TCAAGTAACTAAGGTATTCCTCCTTGTTGAAGCAAATACGAAGAGATTAGTGCACCGCGG
TTGGAGGAGTTTTGCTTCATTACAGACAATACATACACAAGACTAGAAGTAATTAACATG
CAGACTCCATCTCTATCAGTTCTCAGCTAAGATGATTCATTCTTAATAAAAGATTAACAA
AAAATACAATACACTATTTTTTCAGGTACTAAGTATGGAGATTAAAGTTTTAAACTCTCT
TCATTTTCGGTTATCAGTTCCCACCACCAAAACGTTTCTAGGGTAATCAT

Last Updated on 06.12.2018 16:11