Sequences from confirmation amplicon


Confirmation sequences for line 076B07 and hit At5g27530 are shown.
Line ID 076B07
Locus At5g27530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9718523
Primers used for PCR 07cp - 8474, show primer details
   
Sequence Name 88-K108704-0022-076-B07C-07cp
GenBank Accession LN514876 [GenBank]
Sequencing primer gene specific primer, 07cp: 5'-AATTGTGCCTAAATCATTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K108704-0022-076-B07C-07cp
TGGACAGGAGGAAGAATTTTTACCAAAAAAAAGGACTAGGAATGGAGAAAGAATAATCAT
ATGATTCGATGAACGTAAATGCACATTTTTGTGTATATATATACATGATGGAAACACATA
CATAGAGAGACCTAAACTACCTAATACCTATACATTCAATATATTTTCATCATCTTTATC
TACACAATGGTACATATCAAATCAATATATTTTTTAAAATAGTATTTGTTTGCTACTTGA
TAATTCTAACTTTTTCATTCTTGTGAGTTTTAGGAAACCATACTACGTATATCCATATTG
ATAAATTTTATCTGTTTTGGTCTTGTAAACGGCCAAATTTACAATGTACTCAAATTTGGT
GCTGAAGGAGATGGTCAAACCGATGATTCAAATGTAAGTTATTTGTTTTTATCACTAAAA
TATATGAATTTTTGATAAATATTTTCTTAAAATTAATTACTATTTCTTTTTCAAATAGTG
TTGCTACTTTTAATTAACGATATTATATTAAAAGGCTTTTTTACAAGCATGGAACGCTAC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 04-K108703-0022-076-B07C-8409
GenBank Accession LN487953 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108703-0022-076-B07C-8409
AAAAGCCTTTTAATATAATATCGTTAATTAAAAGTAGCAACACTATTTGAAAAAGAAATA
GTAATTAATTTTAAGAAAATATTTATCAAAAATTCATATATTTTAGTGATAAAAACAAAT
AACTTACATTTGAATCATCGGTTTGACCATCTCCTTCAGCACCAAATTTGAGTACATTGT
AAATTTGGCCGTTTACAAGACCAAAACAGATAAAATTTATCAATATGGATATACGTAGTA
TGGTTTCCTAAAACTCACAAGAATGAAAAAGTTAGAATTATCAAGTAGCAAACAAATACT
ATTTTAAAAAATATATTGATTTGATATGTACCATTGTGTAGATAAAGATGATGAAAATAT
ATTGAATGTATAGGTATTAGGTAGTTTAGGTCTCTCTATGTATGTGTTTCCATCATGTAT
ATATATACACAAAAATGTGCATTTACGTTCATCGAATCATATGATTATTCTTTCTCCATT
CCTAGTCCTTTTTTTTGGTAAAAATTCTTCCTCCTGTCCATATACTATATAGGCAGCAAT
CGGGAGTATCTAATTTAGGAAGCAAATGATTAGGCCACAA

Last Updated on 06.12.2018 16:11