Sequences from confirmation amplicon


Confirmation sequences for line 038F12 and hit At5g33290 are shown.
Line ID 038F12
Locus At5g33290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:12559199
Primers used for PCR 04fb - 8474, show primer details
   
Sequence Name 30-K111225-0022-038-F12C-04fb
GenBank Accession LN499775 [GenBank]
Sequencing primer gene specific primer, 04fb: 5'-CTCCTCTTGAACAAAACACAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111225-0022-038-F12C-04fb
CCTCTTGGTCGAGCAACACAACAAACACTATTCTTGCTTCTTCTTCTTCTTCTTCCTCTT
TTTCTGATCACCAAAACCAAAATAAGTCTCCAAGTCCTACAAGTAAAAAAATTGTTATCA
GGGTATGTAATCATTCTTTCTCTCTTTGTTTTTTTCTTTTTCAAGTCTCACAAATAAATA
ATTTTGTATTGATCAAACTCTTTTTCTTTCTAATACTTTTCTTTGTCAAAGTAAATAATA
ATCTTTACTTTTATTTTTACTATGATCAGAAAAGAAGTGGGTTAGACAAAATAGAATCAG
ATTTAGCAAAAGCAAGAGCAGCAATTAAGAAGGCAGCTTCTACACAAAATTATGTCAGCT
CTCTCTATAAAAATCCAGCCGCCTTTCATCAGTTGAGTTTTGCAATTCTTCTTCTTCTTC
TTTTTTTAACCATTTGAGGTAAAAAAAAAGATTTCTTCTTTTATTAAACAGTAATTTAAT
ACTAGTACAGTATTTGACAGTTCCTAGATTTTTAGTCAAATATGATAGCGCTGACAATTT
TAAACCAACATGCAAGCTCCACCGGGTGCAAAGCGGCAGCGGCGGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 14-K111224-0022-038-F12C-8409
GenBank Accession LN492565 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K111224-0022-038-F12C-8409
GCCATTTCATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATG
TTGGTTTAAAATTGTCAGCGCTATCATATTTGACTAAAAATCTAGGAACTGTCAAATACT
GTACTAGTATTAAATTACTGTTTAATAAAAGAAGAAATCTTTTTTTTTACCTCAAATGGT
TAAAAAAAGAAGAAGAAGAAGAATTGCAAAACTCAACTGATGAAAGGCGGCTGGATTTTT
ATAGAGAGAGCTGACATAATTTTGTGTAGAAGCTGCCTTCTTAATTGCTGCTCTTGCTTT
TGCTAAATCTGATTCTATTTTGTCTAACCCACTTCTTTTCTGATCATAGTAAAAATAAAA
GTAAAGATTATTATTTACTTTGACAAAGAAAAGTATTAGAAAGAAAAAGAGTTTGATCAA
TACAAAATTATTTATTTGTGAGACTTGAAAAAGAAAAAAACAAAGAGAGAAAGAATGATT
ACATACCCTGATAACAATTTTTTTACTTGTAGGACTTGGAGACTTATTTTGGTTTTGGTG
ATCAGAAAAAGAGGAAGAAGAAGAAGAAGAAGCAAGAATAGTGTTTGTTGTGTTGCTCTG
ACCAAGAGGGGACAAAGGAGGAGAGGCTGAGACAAAACTAGTGTTTGTTGTGTTTTGTTC
AAGAGGA

Last Updated on 06.12.2018 16:11