Sequences from confirmation amplicon


Confirmation sequences for line 222F02 and hit At5g35840 are shown.
Line ID 222F02
Locus At5g35840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14008686
Primers used for PCR PH27 - 8474, show primer details
   
Sequence Name 01-K017549-022-222-F02C-PH27
GenBank Accession LN486375 [GenBank]
Sequencing primer gene specific primer, PH27: 5'-TAATGGAAACGGAACAAATCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K017549-022-222-F02C-PH27
GCGTGATGACAAACCACCAAGCCCCATAAGTGTCTGCCAGTCTGTAAATCTCTGTTCATC
TCATCACTATCACTACCATTGATAGTTACAGACATGACAAGAGACGCCACTGATCCCATA
TTACTCATATACTGTGCGTGACAACCATGAGGAGCTCTCAAAGTAGATCCAGAAAGACTT
ATTGGCTGTGAGAGACTCTTATCTTGAACGACTTTAACCGGAACCGCTGAACAATCACAA
ATCATCCTAACCTTGTTTCTCATAAACAGAAATCTCGAAGCTTGCGGTATATCAGTAGCG
GAGTAATGCAACCCAAGAAAAGGTTCCATATCTTCCCGGGAGCATTCAGCAATCACTTCC
CCATGGCCATCCTCATGGAACTTATACACCATCCCCCTATCATAACCAGGTAATTCCCTA
ACTTCCTTAACCCAAGCATCCCCCCACCACCACATATAGATGGGGGATATATTCCATTGG
AAATGGCTTCATGGCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGGCCATATGG
AGAAAAAGAAAGAGTAATTACCCATTTTTTTTCCATTCCAAAA
   
Sequence Name 02-K017549-022-222-F02C-8409
GenBank Accession LN486825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K017549-022-222-F02C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCACCATCTATATGTTGTTGTTG
TGTGATGCTTTGGTTAAGGAAGTTAGTGAATTAACTGGTTATGATAGGGTGATGGTGTAT
AAGTTCCATGAGGATGGGCATGGGGAAGTGATTGCTGAATGCTGCCGGGAAGATATGGAA
CCTTATCTTGGGTTGCATTACTCCGCTACTGATATACCGCAAGCTTCGAGATTTCTGTTT
ATGAGAAACAAGGTTAGGATGATTTGTGATTGTTCAGCGGTTCCGGTTAAAGTCGTTCAA
GATAAGAGTCTCTCACAGCCAATAAGTCTTTCTGGATCTACTTTGAGAGCTCCTCATGGT
TGTCACGCACAGTATATGAGTAATATGGGATCAGTGGCGTCTCTTGTCATGTCTGTAACT
ATCAATGGTAGTGATAGTGATGAGATGAACAGAGATTTACAGACTGGCAGACACTTATGG
GGCTTGGTGGTTTGTCATCACGCAAGTCCTAGATTTGTTC

Last Updated on 06.12.2018 16:11