Sequences from confirmation amplicon


Confirmation sequences for line 510C01 and hit At5g37020 are shown.
Line ID 510C01
Locus At5g37020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14630671
Primers used for PCR CB04 - 8474, show primer details
   
Sequence Name 21-K026966-022-510-C01C-8409
GenBank Accession LN495516 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K026966-022-510-C01C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACTATTTCAGGCAGATGTTGAGACGGATG
AAGTCTATGCTCAAATGACACTTCAACCATTGACACCGGTAGATACATTATACATAACAG
GTTTTTGAGTCTGTGAATTAATAACTTAAGCTTTAGGATTGGTGTTGTTTATAGGAGGAG
CAGAAGGAAACATTTGTACCGATTGAGTTGGGGATACCGAGTAAGCAACCTAGTAATTAT
TTTTGTAAGACTCTCACAGCTAGTGATACCAGTACACATGGAGGGTTTTCTGTTCCTAGA
CGTGCTGCTGAGAAAGTGTTTCCTCCATTGGTATTATACTTTTTCTTTTACTTACTTTGC
TATAAATTGTTGTTGAGAAAATATATGTATTCCTTTTAATATCTTGTGTCTTCCCTGTTG
CAGGATTACACACTGCAGCCACCAGCTCAAGAACTGATTGCAAGGGATCTCCATGATGTT
GAA
   
Sequence Name 22-K026966-022-510-C01C-CB04
GenBank Accession LN495962 [GenBank]
Sequencing primer gene specific primer, CB04: 5'-TAAACTTCCATTCAACATCATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K026966-022-510-C01C-CB04
CCTTGCAATCAGTTCTTGAGCTGGTGGCTGCAGTGTGTAATCCTGCAACAGGGAAGACAC
AAGATATTAAAAGGAATACATATATTTTCTCAACAACAATTTATAGCAAAGTAAGTAAAA
GAAAAAGTATAATACCAATGGAGGAAACACTTTCTCAGCAGCACGTCTAGGAACAGAAAA
CCCTCCATGTGTACTGGTATCACTAGCTGTGAGAGTCTTACAAAAATAATTACTAGGTTG
CTTACTCGGTATCCCCAACTCAATCGGTACAAATGTTTCCTTCTGCTCCTCCTATAAACA
ACACCAATCCTAAAGCTTAAGTTATTAATTCACAGACTCAAAAACCTGTTATGTATAATG
TATCTACCGGTGTCAATGGTTGAAGTGTCATTTGAGCATAGACTTCATCCGTCTCAACAT
CTGCCTGAAATAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAATAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACAT

Last Updated on 06.12.2018 16:11