Sequences from confirmation amplicon


Confirmation sequences for line 950G06 and hit At5g38430 are shown.
Line ID 950G06
Locus At5g38430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15385308
Primers used for PCR 50yb - 8474, show primer details
   
Sequence Name 31-K119925-0022-950-G06C-50yb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 50yb: 5'-TTAATATGGGATGGTGTACAACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K119925-0022-950-G06C-50yb
ATTCAAAATTTAAATAGGACGGCGTAAGAAATATTCAATTATTTTTTATTTAATGAAAAT
ATAGACCCGCGGTATACCGCGGATTAATATTTAGTGCTATTGATAAATTTATCTCAACGT
TCAGCTTTGACATTGTGAAATGCATTATGAGTCATGACTCTGTTTATAGATATTCCTGTT
ATAGTATTTATTAACCGAAGTAATGTAAACCAAATTTTAAAAATACATACGAGATAAATA
AAAGGTCCAAAACATTATATTTACGGTCATGCATCTCATTTCTTTTCTTCCAAAAGGAAG
GAGCCAAAGCAACCGATCAAGTGGAGACTAGTAAACCATACACAATCACTCAATTCCTCA
CAAAAGAGAGATAAGATAAGGGTGTCATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCACAAATGTA
   
Sequence Name 15-K119924-0022-950-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119924-0022-950-G06C-8409
ATTGAATATATGACACCCTTATCTTATCTTTCTTTTGTGAGGAAATGAGTGATTGTGTAT
GGTTTACTAGTCTCCACTTGATCGGTTGCTTTGGCTCCTTCCTTTTGGAAGAAAAGAAAT
CAGATGCATGACCGTAAATATAATGTTTTGGACCTTTTATTTATCTTGTATGTATTTTTA
AAATTTGGTTTACATTACTTCGGTTAATAAATACTATAACAGGAATATCTATAAACAGAG
TCATGACTCATAATGCATTTCACAATGTCAAAGCTGAACGTTGAGATAAATTTATCAATA
GCACTAAATATTAATCCGCGGTATACCGCGGGTCTATATTTTCATTAAATAAAAAATAAT
TGAATATTTCTTAGGATGACTTAATAAATTTCTGCTATTGTTTGATTTAAATGTATAATT
CACCGCGAGACAAAATTTTATAACTCAAATTTTAAAGTTTTAAGTTGTATTTGTTTATTT
TGTTAAATGTTTAATATTGTATAATTGTATTTTGATTGTTGTTTCTCGGATTTCACCCGT
TGTACACCATCCCATATTAAAATCGAATCAAACCCGTCAATTCTAGGATTTCACCTGTGG
TAGTATTTAATTGTATAATAATAACTTGATTGTCATTCTAGGATTTCACTCCTAGTTCTA
TCGCAAATTATTATCAACTCAAACCAGTCAATTCTAAAATTTCACCCGTTGTACACCAT

Last Updated on 06.12.2018 16:11