Sequences from confirmation amplicon


Confirmation sequences for line 281G07 and hit At5g39570 are shown.
Line ID 281G07
Locus At5g39570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15843879
Primers used for PCR 17sa - 8474, show primer details
   
Sequence Name 28-K116272-0022-281-G07C-17sa
GenBank Accession LN498998 [GenBank]
Sequencing primer gene specific primer, 17sa: 5'-GATTTGGTCTTTCACCTTGAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116272-0022-281-G07C-17sa
GGGTCGTGGCTCGGGTCGAGAAAGCTACTGTACTCTGTGTTAAGAGCTTCATCACCGTAA
GCAGAAGGCTCATGGATCTGAGTAAATTCAGGTCTCTCGTATTCGAAATCATCATCGACA
CCAGATGAGAGAGGATAACAAGTCTCATCGGAAGGTGGAATCGGACGGCCGTAGATCACC
GTGATGTCGTAACCACCACTGTACGGCGTTGGATCAAACTCATCGAAATCGTCGACGTCG
TTGTCATCTCTGGTATAGTACGGCATCGTTTAAGAAGAAGCTTCTCTGTGTTTGTTTTAC
TCTTTTGGTTTCTTGGATCTGATCAAAATGACAGAAATGAGAATTTATACGCGGGGGAAG
AAGAAGACGAAGAGTTTAAAAACGTTGCGTTTTGAATGAATAATTGTGATGTTCATGATG
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TC
   
Sequence Name 04-K116271-0022-281-G07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116271-0022-281-G07C-8409
CAGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11