Sequences from confirmation amplicon


Confirmation sequences for line 673F07 and hit At5g40280 are shown.
Line ID 673F07
Locus At5g40280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16101395
Primers used for PCR JL05 - 8474, show primer details
   
Sequence Name 35-K026693-022-673-F07C-8409
GenBank Accession LN501686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K026693-022-673-F07C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTTGATTCGGAACCCGC
AGTAGTAACCCGCTTGATTCGTTTGAAGTGTGTAGGGCTCAGACTTGACCGGAGTGGACT
CAATCGGCGAATCTGTCACGGAGGACACGGGGAATCAACGCGGCGGAGAGTGATGGAAGA
GCTTTCAAGCCTAACCGTGAGTCAGCGCGAGCAATTTCTGGTGGAGAACGATGTGTTCGG
GATCTATAATTACTTCGACGCCAGCGACGTTTCTACTCAAAAATACATGTAAGCTCACGG
ATTTGATTTTCTAGTTTTCTTCATGATCTGATGAATTTTAGTAGCGTCGTGAAAGAATTA
TTTTCGTCGATAGATGAATCTTACTGATATGGAAGTTGTTCTATCCTAGGATGGAGATTC
AGCGAGATAAGCAATTGGATTATCTGATGAAAGGCTTAAGGCAGCTTGGTCCGCAGTTTT
CTTCCTTAGATGCTAAGTAAGTGACATGATGCTTGGCTTCTTGTTTTCATGAATTTCTTA
GTACATTTTGTCCAGTGAGAGAGTAAAGCCCCTTTGGATTTTTGCCAATANGATAGAAGT
TTGATTTTGGCTTTTTTGGATTTTGGAACAGT
   
Sequence Name 39-K026693-022-673-F07C-JL05
GenBank Accession LN503223 [GenBank]
Sequencing primer gene specific primer, JL05: 5'-GAAGAATCCAGTAACAAAGCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K026693-022-673-F07C-JL05
GTTCCAACATCCAAAAAAGCCAAAATCAAACTTCTAATCTATTGGCAAAAATCCAAAGGG
GCTTTACTCTCTCACTGGACAAAATGTACTAAGAAATTCATGAAAACAAGAAGCCAAGCA
TCATGTCACTTACTTAGCATCTAAGGAAGAAAACTGCGGACCAAGCTGCCTTAAGCCTTT
CATCAGATAATCCAATTGCTTATCTCGCTGAATCTCCATCCTAGGATAGAACAACTTCCA
TATCAGTAAGATTCATCTATCGACGAAAATAATTCTTTCACGACGCTACTAAAATTCATC
AGATCATGAAGAAAACTAGAAAATCAAATCCGTGAGCTTACATGTATTTTTGAGTAGAAA
CGTCGCTGGCGTCGAAGTAATTATAGATCCCGAACACATCGTTCTCCACCAGAAATTGCT
CGCGCTGACTCACGGTTAGGCTTGAAAGCTCTTCCATCACTCTCCGCCGCGTTGATTCCC
CGTGTCCTCCGTGACAGATTCGCCGATTGAGTCCACTCCGGTCAAGTCTGAGCCCTACAC
ACTTCAAACGAATCAAGCGGGTTACTACTGCGGGTTCCGAATCAAGCAGGATATATTCAA
TTGTAAATGGCTTCATGTC

Last Updated on 06.12.2018 16:11