Sequences from confirmation amplicon


Confirmation sequences for line 315B05 and hit At5g40780 are shown.
Line ID 315B05
Locus At5g40780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16326601
Primers used for PCR UF03 - 8474, show primer details
   
Sequence Name 22-K021971-022-315-B05C-UF03
GenBank Accession LN495931 [GenBank]
Sequencing primer gene specific primer, UF03: 5'-GTTGGATACTACATTTTCGGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K021971-022-315-B05C-UF03
GAAGATAATATTCTCATGTCACTTAAGAAACCGGCGTGGTTAATCGCCACGGCGAACATC
TTCGTCGTGATCCATGTCATTGGTAGTTACCAGGTTGGTTTTTCTAAACAAGAACGTACC
CTTAAATACTCGAGAGAGAACCGTTATTAACTAGGTCTAAACCAAATCTTGGTTTTGTTG
CAGATATATGCAATGCCGGTATTTGATATGATGGAGACTTTATTGGTCAAGAAGCTAAAT
TTCAGACCAACCACAACTCTTCGGTTCTTTGTCCGTAATTTCTACGTTGGTAAGAAAATT
TATTGAAATTCACGTTTATTATTTACTTATGTATATTTACATTTTTAAGGTGTTAGTTGT
TCAAAAAATCTGATGATGAGTTTTTTTGTCATCTTAATTATGGTTTTGTAGCTGCAACTA
TGGAGAAAAAGAAAGAGTAATTACCATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 21-K021971-022-315-B05C-8409
GenBank Accession LN495483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K021971-022-315-B05C-8409
GTAATTACTCTTTCTTTTTCTCCATAGTTGCAGCTACAAAACCATAATTAAGATGACAAA
AAAACTCATCATCAGATTTTTTGAACAACTAACACCTTAAAAATGTAAATATACATAAGT
TAATAATAAACGTGGATTTCAATAAATTTTCTTACCAACGTAGAAATTACGGACAAAGAA
CCGAAGAGTTGTGGTTGGTCTGAAATTTAGCTTCTTGACCAATAAAGTCTCCATCATATC
AAATACCGGCATTGCATATATCTGCAACAAAACCAAGATTTGGTTTAGACCTAGTTAATA
ACGGTTCTCTCTCGAGTATTTAAGGGTACGTTCTTGTTTAGAAAAACCAACCTGGTAACT
ACCAATGACATGGATCACGACGAAGATGTTCGCCGTGGCGATTAACCACGCCGGTTTCTT
AAGTGACATGAGAATATTATCTTCGACTCCGTTCCCGAAAATGT

Last Updated on 06.12.2018 16:11