Sequences from confirmation amplicon


Confirmation sequences for line 099D10 and hit At5g41060 are shown.
Line ID 099D10
Locus At5g41060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16436181
Primers used for PCR 04pm - 8474, show primer details
   
Sequence Name 44-K115064-0022-099-D10C-04pm
GenBank Accession LN505282 [GenBank]
Sequencing primer gene specific primer, 04pm: 5'-TGTGAAAGCTTTAGGTTTTGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K115064-0022-099-D10C-04pm
TTTTCCTATTATTGTGTATAAATTAATTATTAATCCGTTCATGGGCTCAGTTTACTCTGT
TTCCAATCTTGTAAAGCTTAAAAATTGGGACTTGGGTTTGTGTTAGAAGTGTAGCTTTCT
GGGTAGTTGTCTACATTTAATCATGAAATCTTTACCTTTAATCAATACCTTAGGACATTA
TCTGAATATGCTTGCTACATTTATAGGAATGCTTCTTCATACAAAGGTTATATAGTTGAT
GCTGTCTGTATATTGCTTTCAATGTCGAATTTAAATCTGGTTTAGTAGTAACCTAGTGGA
ATTAAACATTTTGAGGTTTGTTCCTTAAATCCTTCTACCCAAAGTGTTAACATATTTTCT
AAATTTGTCCCATTTCTGCAGATATTTTGTCTTCAAGGAAGGTTTATCTTCGGGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
AT
   
Sequence Name 12-K115063-0022-099-D10C-8409
GenBank Accession LN491700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115063-0022-099-D10C-8409
ATTTCATTGAATATATCCTGCCCGAAGATAACCTTCCTTGAAGACAAAATATCTGCAGAA
ATGGGACAAATTTAGAAAATATGTTAACACTTTGGGTAGAAGGATTTAAGGAACAAACCT
CAAAATGTTTAATTCCACTAGGTTACTACTAAACCAGATTTAAATTCGACATTGAAAGCA
ATATACAGACAGCATCAACTATATAACCTTTGTATGAAGAAGCATTCCTATAAATGTAGC
AAGCATATTCAGATAATGTCCTAAGGTATTGATTAAAGGTAAAGATTTCATGATTAAATG
TAGACAACTACCCAGAAAGCTACACTTCTAACACAAACCCAAGTCCCAATTTTTAAGCTT
TACAAGATTGGAAACAGAGTAAACTGAGCCCATGAACGGATTAATAATTAATTTATACAC
AATAATAGGAAAAATAACAGAAAACTAGCTCAAGTATAAACACAGAAGCAAGTAATTCCA
AAACCTAAGCCCTTTCA

Last Updated on 06.12.2018 16:11