Sequences from confirmation amplicon


Confirmation sequences for line 596G12 and hit At5g43180 are shown.
Line ID 596G12
Locus At5g43180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17337770
Primers used for PCR NG13 - 8474, show primer details
   
Sequence Name 68-K104216-0022-596-G12C-NG13
GenBank Accession LN512007 [GenBank]
Sequencing primer gene specific primer, NG13: 5'-ATTATTCCGTTTCCTCGAAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K104216-0022-596-G12C-NG13
TTCAAGGTATTAATAGTTGTTAAGAAAAAAAATACAAAGACATAAGGGGGTTGATTGTTT
CGCAAAGTTTTGTCTTTGTGTTAACATGTGAATTACTTTTCGATTTTTCACTAAATACAT
CTAAACGCAAACACCATTTTATTCCGCTTGCGTTTATACAACTAATAAAGATTTTTTTTG
GTAAAAACAATTAAGGATCTAGATTGTTTATTAATATGTAAAACAAAAGTGTGTATGCAT
GTTTACGTATTCATATTTATCATCTTTTGAAAATTTTGAGAGCAAACGTAAAAGAACAAT
AGAAATGTTTCTAAAATTTTGTATATTTGCCTAAAGTTTTAAAATAATTTTTATATCATA
AGTTTATATACATATAAGCGCAAAACAAAGTGTACGTAAACAAACTTCTAAAAACAGAAA
AGAAGAAGAACCTCTTTGATGTCACGGAACCAAGATTTGCGTTTTGAGGAATCAATGCCA
AGTGAAGTACGAGAAGGATTGTGCTTGAAGGTGTTCCATAGAAAGACATGGTATCCCACA
GCTATCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATT
   
Sequence Name 28-K104217-0022-596-G12C-8409
GenBank Accession LN498822 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104217-0022-596-G12C-8409
TTACAATTGAATATATCCTGTTGATAGCTGTGGGATACCATGTCTTTCTATGGAACACCT
TCAAGCACAATCCTTCTCGTACTTCACTTGGCATTGATTCCTCAAAACGCAAATCTTGGT
TCCGTGACATCAAAGAGGTTCTTCTTCTTTTCTGTTTTTAGAAGTTTGTTTACGTACACT
TTGTTTTGCGCTTATATGTATATAAACTTATGATATAAAAATTATTTTAAAACTTTAGGC
AAATATACAAAATTTTAGAAACATTTCTATTGTTCTTTTACGTTTGCTCTCAAAATTTTC
AAAAGATGATAAATATGAATACGTAAACATGCATACACACTTTTGTTTTACATATTAATA
AACAATCTAGATCCTTAATTGTTTTTACCAAAAAAAATCTTTATTAGTTGTATAAACGCA
AGCGGAATAAAATGGTGTTTGCGTTTAGATGTATTTAGTGAAAAATCGAAAAGTAATTCA
CATGTTAACACAAAGACAAAACTTTGCGAAACAATCAACCCCCTTATGTCTTTGTATTTT
TTTTCTTAACAACTATNAAATACCTTTGAACAATCTATAGGTATTACTTTAAAAAAATCT
TC
Primers used for PCR NE70 - 8474, show primer details
   
Sequence Name 69-K104216-0022-596-G12C-NE70
GenBank Accession LN512197 [GenBank]
Sequencing primer gene specific primer, NE70: 5'-AAAAACACTACAGAGGCATTAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K104216-0022-596-G12C-NE70
AGCAACTTTTTCTAGCGTTTACATGTGAGTTATTTTTTTTCCTTTCCATTTTCCTCTAAA
TATATTTGTTAAATGTAAACACGACGATATGATTATTCCGTTTCCTCGAAGATTTTTTTA
AAGTAATACCTATAGATTGTTCAAAGGTATTTAATAGTTGTTAAGAAAAAAAATACAAAG
ACATAAGGGGGTTGATTGTTTCGCAAAGTTTTGTCTTTGTGTTAACATGTGAATTACTTT
TCGATTTTTCACTAAATACATCTAAACGCAAACACCATTTTATTCCGCTTGCGTTTATAC
AACTAATAAAGATTTTTTTTGGTAAAAACAATTAAGGATCTAGATTGTTTATTAATATGT
AAAACAAAAGTGTGTATGCATGTTTACGTATTCATATTTATCATCTTTTGAAAATTTTGA
GAGCAAACGTAAAAGAACAATAGAAATGTTTCTAAAATTTTGTATATNTGCCTAAAGTTT
TAAAATAATTTTTATATCANAAGTTTATATACATATNAGCGCAAAACAGAGTGTACGTAA
ACAAACTTCTAAAAACAGAAAAGAAGAAGAACCTCTNTGATGTCACGGAACCAAAATTTG
CGTTTTGAGGA
   
Sequence Name 29-K104217-0022-596-G12C-OriL
GenBank Accession LN499266 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104217-0022-596-G12C-OriL
TTGAATATATCCTGTTGATAGCTGTGGGATACCATGTCTTTCTATGGAACACCTTCAAGC
ACAATCCTTCTCGTACTTCACTTGGCATTGATTCCTCAAAACGCAAATCTTGGTTCCGTG
ACATCAAAGAGGTTCTTCTTCTTTTCTGTTTTTAGAAGTTTGTTTACGTACACTTTGTTT
TGCGCTTATATGTATATAAACTTATGATATAAAAATTATTTTAAAACTTTAGGCAAATAT
ACAAAATTTTAGAAACATTTCTATTGTTCTTTTACGTTTGCTCTCAAAATTTTCAAAAGA
TGATAAATATGAATACGTAAACATGCATACACACTTTTGTTTTACATATTAATAAACAAT
CTAGATCCTTAATTGTTTTTACCAAAAAAAATCTTTATTAGTTGTATAAACGCAAGCGGA
ATAAAATGGTGTTTGCGTTTAGATGTATTTAGTGAAAAATCGAAAAGTAATTCACATGTT
AACACAAAGACAAAACTTTGCGAAACAATCAACCCCCTTATGTCTTTGTATTTTTTTTCT
TAACAACTATNAAATACCTTTGAACAATCTATAGGTATTACTTTAAAAAAATCTTCGAGG
AAACGGAATAATCATATCGTCGTGTTTACATTTAACAAATATATNTAGAGGAAAATGGAA
AGGAAAAAAAATAACTCACATGTAAACGCTAAGAAAAAGTTTTGCTTTTAGGAGTAAATC
AATTAAATC

Last Updated on 06.12.2018 16:11