Sequences from confirmation amplicon


Confirmation sequences for line 297G08 and hit At5g45250 are shown.
Line ID 297G08
Locus At5g45250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18325134
Primers used for PCR AC20 - 8474, show primer details
   
Sequence Name 17-K027789-022-297-G08C-8409
GenBank Accession LN493714 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K027789-022-297-G08C-8409
CTTTCGGAGTGTGGTTTTACCAATTCCGGGCATCCCAACAACTCCAATGATACGAGTTCC
CTTGTATTTATCACGATCCAACTTCTCTTCCAAATCTTTTAACCGTTGTTCGTTTCCAAA
AGTCTCATGCTTTTTGTCTCCTGAGGAAGTTCCAGCGTTGCTATTACCTAAAGCACCCAC
GACGGCGTTGTGACTTCCCTCCGGTGGTATTCCGGTCAACGCTGTCTTCACCGCCTTCAC
AATTTCATTGACTTTCTCACTCTCCACACTGCAATTTCAAGGCTAATGAGATCATGACCT
CCCTAAGTTCCATAAAAGGTTCTAGAAGTATTCATAGATCTGTCTACAATTGTAATCTAA
TCACTACTGTGTCTAGGTTTGTAACTGAGTCAAAAAAAATAAAAAAAAATGGTACCTTTT
CTTGTCAATGATGATGCCCATAATGTTAGGAATCAAGTTAAAAGCTTCCTTCCATTTCTT
TTTCCTCTCATC
   
Sequence Name 18-K027789-022-297-G08C-AC20
GenBank Accession LN494162 [GenBank]
Sequencing primer gene specific primer, AC20: 5'-GAGTATGGCTAAGGGTGATGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K027789-022-297-G08C-AC20
GCTTTTACTTGATTCCAACATTATGGGCATCATCATTGACAAGAAAAGGTACCATTTTTT
TTTATTTTTTTTGACTCAGTTACAAACCTAGACACAGTAGTGATTAGATTACAATTGTAG
ACAGATCTATGAATACTTCTAGAACCTTTTATGGAACTTAGGGAGGTCATGATCTCATTA
GCCTTGAAATTGCAGTGTGGAGAGTGAGAAAGTCAATGAAATTGTGAAGGCGGTGAAGAC
AGCGTTGACCGGAATACCACCGGAGGGAAGTCACAACGCCGTCGTGGGTGCTTTAGGTAA
TAGCAACGCTGGAACTTCCTCAGGAGACAAAAAGCATGAGACTTTTGGAAACGAACAACG
GTTAAAAGATTTGGAAGAGAAGTTGGATCGTGATAAATACAAGGGAACTCGTATCATTGG
AGTTGTTGGGATGCCCGGAATTGGTAAAACCACACTCCTGAAAGATGATGGATCAGCAAT
ACAATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11