Sequences from confirmation amplicon


Confirmation sequences for line 208E01 and hit At5g46600 are shown.
Line ID 208E01
Locus At5g46600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18908276
Primers used for PCR 15uh - 8474, show primer details
   
Sequence Name 33-K117093-0022-208-E01C-15uh
GenBank Accession LN501156 [GenBank]
Sequencing primer gene specific primer, 15uh: 5'-TTTTAAGACTCCAAGGTCTATCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117093-0022-208-E01C-15uh
CCGGAGAATCTGCAAGTTTTATCAGAACTCTCCGAAAGCATTCAAAACCGACGTCATTGC
TCGTCGGAGATTCTCTCCGACAGTCTCGAAGCGGCATTAAAAGATCTCAACTCCACAATC
AAGTCACAACCTAAGCTCTTCTTAGGCTCTAACCTTCATAGTAACATGGATATATTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 17-K117092-0022-208-E01C-8409
GenBank Accession LN494028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117092-0022-208-E01C-8409
TTACTATGAAGGTTAGAGCCTAAGAAGAGCTTAGGTTGTGACTTGATTGTGGAGTTGAGA
TCTTTTAATGCCGCTTCGAGACTGTCGGAGAGAATCTCCGACGAGCAATGACGTCGGTTT
TGAATGCTTTCGGAGAGTTCTGATAAAACTTTGCAGATTTCTCCGGCTAGTCTAACACAA
GGATCTTTGAAGAGGACACGGATAGACCTTGGAGTCTTAAAAAATCCTNTGTGTTGACTG
CCGGAGAAATCTGCAAAGTTTTATCAGCCTCTCCGA

Last Updated on 06.12.2018 16:11