Sequences from confirmation amplicon


Confirmation sequences for line 420C05 and hit At5g47720 are shown.
Line ID 420C05
Locus At5g47720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19332131
Primers used for PCR AM07 - 8474, show primer details
   
Sequence Name 14-K105747-0022-420-C05C-AM07
GenBank Accession LN492508 [GenBank]
Sequencing primer gene specific primer, AM07: 5'-CAAGTTTGAACATGAGTGACCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K105747-0022-420-C05C-AM07
GCATATTTCTCCACAAACTCCCATTCCAAGTCATTATAGACATCCCAAAGTCCATCTTTC
ATCATACCATCAACAACAGTATCATGACCTAATCGAGAACCCCTTCTGCTAACAGAAATC
AGAGTAAAGAATAAACGAGATGATAAAAACAGATGGAATATGCCTCTCTTGGGTTTGTTT
CTACAAGTTTGAGTTAGGAAACTCAAAAAGCACCTTGCGTCCGGGAGGTACTTAGGTACG
TTTGACATGCTCTCCATCCCACCAGCAACAACAATATCATTCAAGCCGAGCTGGATACTT
TGAGACGCTAGCATTACAGCTGAGGTTACAAGTGAAGTAAAAGCAGTGATATATACACCG
TTCGAATGATCATCTGGAGTAGAAGAAGAGTAATAAAAGGTTATTACATTTCATTCCTGC
AGCACAAACTTTGTTGATAGTGGTGCAGATCACAGAATAGGGAATCCCAGCACCAAGTGC
AGCCTGTCTTGCTGGTGCTTGCCCAAGATTGGCAGTTAAGACATTGCCAAAGAAGACCTC
TTCCACAAGGGCCGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTC
   
Sequence Name 30-K105748-0022-420-C05C-8409
GenBank Accession LN499721 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K105748-0022-420-C05C-8409
CATTTACAATTGAATATTTCCGGCCCTTGTGGAAGAGGTCTTCTTTGGCAATGTCTTAAC
TGCCAATCTTGGGCAAGCACCAGCAAGACAGGCTGCACTTGGTGCTGGGATTCCCTATTC
TGTGATCTGCACCACTATCAACAAAGTTTGTGCTGCAGGAATGAAATGTAATAACCTTTT
ATTACTCTTCTTCTACTCCAGATGATCATTCGAACGGTGTATATATCACTGCTTTTACTT
CACTTGTAACCTCAGCTGTAATGCTAGCGTCTCAAAGTATCCAGCTCGGCTTGAATGATA
TTGTTGTTGCTGGTGGGATGGAGAGCATGTCAAACGTACCTAAGTACCTCCCGGACGCAA
GGTGCTTTTTGAGTTTCCTAACTCAAACTTGTAGAAACAAACCCAAGAGAGGCATATTCC
ATCTGTTTTTATCATCTCGTTTATTCTTTACTCTGATTTCTGTTAGCAGAAGGGGTTCTC
GATTAGGTCATGATACTGTTGTTGATGGTATGATGAAAGATGGACTTTGGGATGTCTATA
ATGACTTTGGAATGGGAGTTTGTGGAGAAATATGCGCTGACCAGTACCGTATTACAAGAG
AAGAACAGGTCACTCATGTTCAAACTT

Last Updated on 06.12.2018 16:11