Sequences from confirmation amplicon


Confirmation sequences for line 399A12 and hit At5g49630 are shown.
Line ID 399A12
Locus At5g49630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20146124
Primers used for PCR RK83 - 8474, show primer details
   
Sequence Name 03-K021642-022-399-A12C-8409
GenBank Accession LN487295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K021642-022-399-A12C-8409
ATTCGGACATGTAATTTCTTTTCTTTTTCGTCTTTATTAATGTTTCACGCTTGCGTCTAG
AATTGTTTAACGTGCTAAAATAAATCTTCTTTGAAACATAATCATAATTTTGCATGCCAA
CACATACAATACAATGGACTAGCTGAGACTTATTTACTTGCCCGTTATATATACACATCT
ATGTGATCATGCTGTCTGAATCATAGCGTATATAAGATTTAGAGAGATAACGATGAGTTA
CCAGTTCTTTTGTCGCGGCCATCCTCGTCAAAGTTTTTGTTAGTATCGCCAATTTCATGC
TCCGGGAAGCTCTGTTCAACGAACATGCTCTTCTTCTTCTCCATCTATCGATTGTTTTTG
AGCTTAGCTATGATAAAATAAGAAGAAGAGGCCAAAGGTAGTATGTATGGAGTGTCAAGG
ACTCTTCTCCACTTAAATAGACACACCACATTTCACACCTAATCCCTTTCTCTCTTTTAT
TTTATTTTAAATGTCATCTTTTATATTGTAGTGTTCATTTTTCACACCTTTCATTATTGT
CTGTCTCTGTAATTAATATATTTATTATTTTACGTTATGATTTTATGAATGAGATTCATT
AATGTCGAATAGATNGCTTCGATCGATTATATATATGGGATAGGCTCAGACCCATT
   
Sequence Name 04-K021642-022-399-A12C-RK83
GenBank Accession LN487737 [GenBank]
Sequencing primer gene specific primer, RK83: 5'-AGTCAACTTTCAACAAATGGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K021642-022-399-A12C-RK83
TATCGATCGAGCATCTATTCGACATTAATGAATCTCATTCATAAAATCATAACGTAAAAT
AATAAATATATTAATTACAGAGACAGACAATAATGAAAGGTGTGAAAAATGAACACTACA
ATATAAAAGATGACATTTAAAATAAAATAAAAGAGAGAAAGGGATTAGGTGTGAAATGTG
GTGTGTCTATTTAAGTGGAGAAGAGTCCTTGACACTCCATACATACTACCTTTGGCCTCT
TCTTCTTATTTTATCATAGCTAAGCTCAAAAACAATCGATAGATGGAGAAGAAGAAGAGC
ATGTTCGTTGAACAGAGCTTCCCGGAGCATGAAATTGGCGATACTAACAAAAACTTTGAC
GAGGATGGCCGCGACAAAAGAACTGGTAACTCATCGTTATCTCTCTAAATCTTATATACG
CTATGATTCAGACAGCATGATCACATAGATGTGTATATATAACGGGCAAGTAAATAAGTC
TCAGCTAGTCCATTGTATTGTATGTGTTGGCATGCAAAATTATGATTATGTTTCAAAGAA
GATTTATTTTAGCACGTTAAACAATTCTAGACGCAAGCGTGAAACATTAATAAAAGACGA
AAAAGAAAAAGAAATCTACATGGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACG
TGTCT

Last Updated on 06.12.2018 16:11