Sequences from confirmation amplicon


Confirmation sequences for line 633B04 and hit At5g49890 are shown.
Line ID 633B04
Locus At5g49890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20289815
Primers used for PCR BB76 - 8474, show primer details
   
Sequence Name 33-K104125-0022-633-B04C-BB76
GenBank Accession LN500973 [GenBank]
Sequencing primer gene specific primer, BB76: 5'-CTCTGGAGGACATTCTTCACTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104125-0022-633-B04C-BB76
AGTTTGATTGAGTTCTGTCGCTCTGGGAGATGTGGACTGTTTGGGAAAGGAGGTCTCATC
ATGTTTGATGTAAACTCGGGACCAGTGCTATATAGCACTCCAGATTTGCTAGCAATAGTA
TTCCTTGGAGTTATTGGTGGTGTGCTTGGAAGCCTTTACAACTACCTTGTGGACAAAGTC
CTCCGCACATATNGCATTATAAACGAGTACGTCATTTCTCAACAAGTTAACTCTTTCATA
AAGTCATTTTCCAACGAGTTACTCTTTCATAATATCATTTCTTTGCCTCTTTTCGATTTT
TCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAAATGTCCGCACGGTTATAAT
   
Sequence Name 49-K104126-0022-633-B04C-8409
GenBank Accession LN506824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104126-0022-633-B04C-8409
AAGCCATTTACACTTGAATATATCCTGAAAAATCGAAAAGAGGCAAAGAAATGATATTAT
GAAAGAGTAACTCGTTGGAAAATGACTTTATGAAAGAGTTAACTTGTTGAGAAATGACGT
ACTCGTTTATAATGCTATATGTGCGGAGGACTTTGTCCACAAGGTAGTTGTAAAGGCTTC
CAAGCACACCACCAATAACTCCAAGGAATACTATTGCTAGCAAATCTGGAGTGCTATATA
GCACTGGTCCCGAGTTTACATCAAACATGATGAGACCTCCTTTCCCAAACAGTCCACATC
TCCCAGAGCGACAGAACTCAATCAAACTTCGTAAGACCACTGCTACAACTGCGGTAGTGA
AGAATGCCCTCCAG

Last Updated on 06.12.2018 16:11