Sequences from confirmation amplicon


Confirmation sequences for line 160F01 and hit At5g50360 are shown.
Line ID 160F01
Locus At5g50360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20505774
Primers used for PCR 38mm - 8474, show primer details
   
Sequence Name 22-K114570-0022-160-F01C-38mm
GenBank Accession LN496217 [GenBank]
Sequencing primer gene specific primer, 38mm: 5'-GGAAATGATATGTCGAAGGTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114570-0022-160-F01C-38mm
ATTCTGGTGGATCTCCTGCTTCACCGACGGAGAAGAATGGATCGGTGATAACTAACGTTT
CGTCGAGATCACGGAGAGGTAAGAGAAAATATTCAAAGGAGAATAATAGTAGCAGTACCG
GATCCGTTAATAGTAACGGTAACAGCAAACGACAGAGAAACGATGAGACGAAGAACGGAT
CTGGTGGTGGTAGAGAGATCGTGACGTTGCCTCTTCTTCCAGAGACTCCTGAGAAGAAAG
ACTCGCCGTTGAAGGCTAAGGCGGCGCCGGAGTTAGGTGCGGCGGCGTTGTGGCTGAGTT
TTAATGACGGAGCTAGTTATAACAGACGTTATCAGACAGAGTTAATGACGGAAACGGTTG
TGTCGTCGTTGTTGACGGTGGAGTGTGTAACGGAGAGATTGATGGAGGGAGAGTATGAGT
TAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGCAGC
ATTA
   
Sequence Name 09-K114569-0022-160-F01C-8409
GenBank Accession LN490287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114569-0022-160-F01C-8409
ATCTACTCAACTCTCCCTCCATAATCTCTCCGTTACCACTCCACCGTCAACAACGACGAC
ACAACCGTTTCCGTCATTAACTCTGTCTGATAACGTCTGTTATAACTAGCTCCGTCATTA
AAACTCAGCCACAACGCCGCCGCACCTAACTCCGGCGCCGCCTTAGCCTTCAACGGCGAG
TCTTTCTTCTCAGGAGTCTCTGGAAGAAGAGGCAACGTCACGATCTCTCTACCACCACCA
GATCCGTTCTTCGTCTCATCGTTTCTCTGTCGTTTGCTGTTACCGTTACTATTAACGGAT
CCGGTACTGCTACTATTATTCTCCTTTGAATATTTTCTCTTACCTCTCCGTGATCTCGAC
GAAACGTTAGTTATCACCGATCCATTCTTCTCCGTCGGTGAAGCAGGAGATCCACCAGAA
TCAGGTCTCGGTGCAATCGGACGGTACCGGAGCATAATCCGATCAACCTTCGACTATTCA
TTTCC

Last Updated on 06.12.2018 16:11