Sequences from confirmation amplicon


Confirmation sequences for line 203A02 and hit At5g51060 are shown.
Line ID 203A02
Locus At5g51060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20758684
Primers used for PCR BF51 - 8474, show primer details
   
Sequence Name 09-K029909-022-203-A02C-8409
GenBank Accession LN490049 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K029909-022-203-A02C-8409
TTTTGGCATGTGAACAATCAAAACCAAGATTAAGTAGAAGTAAATGAATTGAAAAATCAT
ATGCCTAACGAAACATATTAATTTGGCATGTGAACACTTGCAAGTATATTCGTACACCTA
GATGAGCTGGATCTATATTATATCTACATGTGATGTGCCCATATAACCCAAGCTATAAGA
TAGGAAACTACGAAGACCATTTAAAAAGTCAGCATCTCTTTATTTTATTTTTATTTAGAA
AAAGGAACTTTATAAAGTAAATCGACAATAAAATATGAATTGACGTGATGCAAAATATTT
TTTAAAAACGTAGAATGTTGACCTAGCCTAATTAATTATCCGATCACCTTGCAAAGGTTT
TTCAAGGAAAAGGTTGAGAAATAAGATATTGCAACCCAAAGATTTACGCGGTCACATGGA
GAAGCTAAAAAACATCGTGCTTGCATGATATAACCAAATAATGCGTCTCAATCACCTAAA
TAGAATAGTGATATTTTGGTTAGGTACATAGTTTGTTTTAGTTGTGGTCTCCCAC
   
Sequence Name 09-K029909-022-203-A02C-BF51
GenBank Accession LN490050 [GenBank]
Sequencing primer gene specific primer, BF51: 5'-TTATAATAAAGCGGTGTGGGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K029909-022-203-A02C-BF51
CAAATATCCTATTCTATTTAGGTGATTGAGACGCATTATTTGGTTATATCATGCAAGCAC
GATGTTTTTTAGCTTCTCCATGTGACCGCGTAAATCTTTGGGTTGCAATATCTTATTTCT
CAACCTTTTCCTTGAAAAACCTTTGCAAGGTGATCGGATAATTAATTAGGCTAGGTCAAC
ATTCTACGTTTTTAAAAAATATTTTGCATCACGTCAATTCATATTTTATTGTCGATTTAC
TTTATAAAGTTCCTTTTTCTAAATAAAAATAAAATAAAGAGATGCTGACTTTTTAAATGG
TCTTCGTAGTTTCCTATCTTATAGCTTGGGTTATATGGGCACATCACATGTAGATATAAT
ATAGATCCAGCTCATCTAGGTGTACGAATATACTTGCAAGTGTTCACATGCCAAATTAAT
ATGTTTCGTTAGGCATATGATTTTTCAATTCATTTACTTCTACTTAATCTTGGTTTTGAT
TGTTCACATGCCAAAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATT

Last Updated on 06.12.2018 16:11