Sequences from confirmation amplicon


Confirmation sequences for line 032F01 and hit At5g51545 are shown.
Line ID 032F01
Locus At5g51545 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20937334
Primers used for PCR 03xk - 8474, show primer details
   
Sequence Name 74-K119123-0022-032-F01C-03xk
GenBank Accession LN513156 [GenBank]
Sequencing primer gene specific primer, 03xk: 5'-TTAGTGTTCATGGTGAAGGCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K119123-0022-032-F01C-03xk
CGGAACAACAATACTTTTAACACAAAATGCTCTTTATAATAGTTTATTGAGAAACGGCAA
GTAGTTTGGGTTTGAGTAATGCGATGTCGTTTTCGAGTGAAATATAATAATCCATGACTG
GATAATGGCATCAACTAAAAATGGCGCTACAAATCCACTCTCCGTGTTCCTTCTCCACGA
GACCCTATCATCTCTTCTTCACTACCAGAAACCCTAGATTCGCAATCAAATGTCAAAATT
CCCAAATCGAATCCGATACAACAGAGGATCCATCTCGTTCAAAGAATTCAAGCTCTTCCG
GCGTAGGATTCGGTTCACCGGCGTCTTCTTCTTCGCCGGCTAAGAAATTGAGTGCGGCTA
CATCGGGAAACAAGAAAGGGAAAGGTAAGAGAGAGGTGAATCGGCGTGCTCCTGTGGAGA
AACCTGTGTTCATGAGCGAAGAAGGAGCGGCCAAGGCAGAGGAGCAGAGACAAAACGAAA
ACGCGTTTCTTCTTACTTGGCTTGGGCTTGGTATCGTCATCCTCATCGAAGGCATTATTC
TCGCTGCTTCAGGTATTGAATCTGGGCATTTGCATAAAACTTGGTTTGGTTTGTTAACAG
TCGTTAGTGATATGGATCAAATTGCTTAAATCTGTGATTTAGCTAGAGAGATAATGAATG
ATCAGAAGAGGATACTTCTCATTGCCTCTTCTTAGTAGTCTTTGAATATACAGTTATTGA
AATCTCCAATTCAATTCAATAGATGTGTTGAGAGGATAAACAGTTATTGAAATCTCCTTT
GT
   
Sequence Name 51-K119111-0022-032-F01C-8409
GenBank Accession LN507656 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K119111-0022-032-F01C-8409
ACTAAGGTCCGACCATTACACAAACTCCGCTTGCAACACACTAATTTAACATCAATCACG
AGAAAGCTCAAATATCCATGGNTTGTATAGTATCACTCTTGACCCTTCATTTTCTCATTC
TGTATGTACTTCAGAACTCCATATGCAGTTGTCCCAGCTACGAATAACACAACCGATGGA
GTGAACACTGGATACACATACTTCACAAACAACTTATCCAACTCTTCTGGGAGGAATCCT
GCAGAAGCCAACACAAACACACAAAGGAGATTTCAATAACTGTTTATCCTCTCAACACAT
CTATTGAATTGAATTGGAGATTTCAATAACTGTATATTCAAAGACTACTAAGAAGAGGCA
ATGAGAAGTATCCTCTTCTGATCATTCATTATCTCTCTAGCTAAATCACAGATTTAAGCA
ATTTGATCCATATCACTAACGACTGTTAACAAACCAAACCAAGTTTTATGCAAATGCCCA
GATTCAATACCTGAAGCAGCGAGAATAATGCCTTCGATGAGGATGACGATACCAAGCCCA
AGCCAAGTAAGAAGAAACGCGTTTTCGTTTTGTCTCTGCTCCTCTGCCTTGGCCGCTCCT
TCTTCGCTCATGAACACAGGTTTCTCCACAGGAGCACGCCGATTCACCTCTCTCTTACCT
TTCCCTTTCTTGTTTCCCGATGTAGCCGCACTCAATTTCTTAGCCGGCGAAGAAGAAGAC
GCCGGTGAACCGAATCCTACGCCGGA

Last Updated on 06.12.2018 16:11