Sequences from confirmation amplicon


Confirmation sequences for line 196B10 and hit At5g53200 are shown.
Line ID 196B10
Locus At5g53200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21583872
Primers used for PCR BL82 - 8474, show primer details
   
Sequence Name 50-K105140-0022-196-B10C-BL82
GenBank Accession LN507163 [GenBank]
Sequencing primer gene specific primer, BL82: 5'-CGATATACCGCGGATTAAAATCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105140-0022-196-B10C-BL82
AAACCTAACCGCATGGATTAAAGTTGATTACCAAATGAAACCTATACTATAAAAATTATA
AATGAAATAACAACAAAAAGAAAACAATAGATGATGAATTCTTCAAACTAACAAGAACCA
CTACTATACATCTATTATAATTTTTTTATTTTCTTTTTTCGTTGGCTTAAAAACAAAAAC
ACTAGGAAGGATAGATAGAAAAGCGAGGACGGTGAGGCTTGGTATGTTTGTGGGAAGATG
AGTGAAGCTGGCGTCGTTTATCAGCAAAGCCTTCACTGTTTCTCATTATCCAATATAGAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 34-K105141-0022-196-B10C-8409
GenBank Accession LN501411 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105141-0022-196-B10C-8409
GCTTTGCTGATAAACGACGCCAGCTTCACTCATCTTCCCACAAACATACCAAGCCTCACC
GTCCTCGCTTTTCTATCTATCCTTCCTAGTGTTTTTGTTTTTAAGCCAACGAAAAAAGAA
AATAAAAAAATTATAATAGATGTATAGTAGTGGTTCTTGTTAGTTTGAAGAATTCATCAT
CTATTGTTTTCTTTTTGTTGTTATTTCATTTATAATTTTTATAGTATAGGTTTCATTTGG
TAATCAACTTTAATCCATGCGGTTAGGTTTTTTTATTTTCTCGTCTACGACTTTTATATC
CACAACTAGATTTTAATCCGCGGGTATATC

Last Updated on 06.12.2018 16:11