Sequences from confirmation amplicon


Confirmation sequences for line 105F05 and hit At5g54470 are shown.
Line ID 105F05
Locus At5g54470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22114788
Primers used for PCR LD01 - 8474, show primer details
   
Sequence Name 19-K023619-022-105-F05C-8409
GenBank Accession LN494588 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K023619-022-105-F05C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATCAAAGAAGATAAAGAAAAAGATTCATA
TTCATACATCGGAGAGGTTAAGATCCAAATCAAGTCTCGTCCTTTTCACCACCTGATCTC
CTTCACCACTACTAACCGAAGACGACGAACTCACCACCGGAAGTTCTTGCTCCACCGCAG
CCGGAACAACCTGATTCTCCACTTCTTCTTCTTCTTCATCCTCATCGCTTTCCTCATCAT
AAGACTCAGCTCCGTCGTTACAACCAATGATCTCTTCTTCTTGGTTAAGATTCTGATCCC
TCCCGGCGAGGGAGCTGTTGTTATTCTTCTTACGAGCTAAACAAGACTCACAGATAGAAA
CAGTTGGGCCAAGATTCAGCCCAGAAGCTTTCCAAGGCGTGTGTGACTGACACGCGCTAC
ATAGAAGACA
   
Sequence Name 20-K023619-022-105-F05C-LD01
GenBank Accession LN495044 [GenBank]
Sequencing primer gene specific primer, LD01: 5'-AAACACATGCGTTGTCTTCTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K023619-022-105-F05C-LD01
AGCGCGTGTCAGTCACACACGCCTTGGAAAGCTTCTGGGCTGAATCTTGGCCCAACTGTT
TCTATCTGTGAGTCTTGTTTAGCTCGTAAGAAGAATAACAACAGCTCCCTCGCCGGGAGG
GATCAGAATCTTAACCAAGAAGAAGAGATCATTGGTTGTAACGACGGAGCTGAGTCTTAT
GATGAGGAAAGCGATGAGGATGAAGAAGAAGAAGAAGTGGAGAATCAGGTTGTTCCGGCT
GCGGTGGAGCAAGAACTTCCGGTGGTGAGTTCGTCGTCTTCGGTTAGTAGTGGTGAAGGA
GATCAGGTGGTGAAAAGGACGAGACTTGATTTGGATCTTAACCTCTCCGATGTATGAATA
TGAATCTTTTTCTTTATCTTCTTTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11