Sequences from confirmation amplicon


Confirmation sequences for line 596G06 and hit At5g56530 are shown.
Line ID 596G06
Locus At5g56530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22890422
Primers used for PCR 42ym - 8474, show primer details
   
Sequence Name 36-K120479-0022-596-G06C-42ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 42ym: 5'-CACTACTTCTCCACCCCACTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K120479-0022-596-G06C-42ym
AGCTTGGCCAGTATCCCAACACATACCCNGTCTCAAACTGCATCCACCAGTGCCCTTCTT
TTGGATCCTACAAGAACAATGTCCCCGAGTTATCAGTTTTTATTAATCAGACACACTCAC
ATATCCATAAAGATAACATCATTGAGGGCAAAATGTTGAGGTTCAAGAATTTCATGAAAA
AGATGGTTCTTTAGAGAAGCTCAAGAAATGGATACCAGGTTGATTCATCCATAAAGGTTG
AGACTTTTTACCTTCCAAATGGTAATACTTATATCGTATTGCGGGTTATGGAAGCCAGAT
ACCGGAGAGATACTTGCTCCCATTGCTATTTGACTGTTGATTTGTATGAAACCCGAACAA
AGGAGATTGTAGCAGCCCGTAGCTTGATAAGCATCGCTCTTTTATGTTCCAAGAAGACAT
ATCCAACAAAATATGTTAGCTCAAAGGGTATATACTATACTCTCAGTGAAGAAAGTTTTT
CAGTTTTTTTTCTGAGCTTACCGTCCAGTATGTGAAAAGCCTTGTGTTGTTATCGCCATA
TAAATCTGGACTAACCTGTATCGATATATATGTCAGTGAAGCTAAGAACACAAGTGTAAT
TGAGATTAAGCTAAACTGCTATGACATTATCTAGGTGCTTTACCTGCCAACCAGCTTCAA
TGCTGTTGAGATCTTGACCAAAAGAACCTCCNAGAATCCATAACTGATCAATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
   
Sequence Name 12-K120478-0022-596-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K120478-0022-596-G06C-8409
GCCATTTACATTGAATTGATCAGTTATGGATTCTTGGAGGTTCTTTTGGTCAAGATCTCA
ACAGCATTGAAGCTGGTTGGCAGGTAAAGCACCTAGATAATGTCATAGCAGTTTAGCTTA
ATCTCAATTACACTTGTGTTCTTAGCTTCACTGACATATATATCGATACAGGTTAGTCCA
GATTTATATGGCGATAACAACACAAGGCTTTTCACATACTGGACGGTAAGCTCAGAAAAA
AAACTGAAAAACTTTCTTCACTGAGAGTATAGTATATACCCTTTGAGCTAACATATTTTG
TTGGATATGTCTTCTTGGAACATAAAAGAGCGATGCTTATCAAGCTACGGGCTGCTACAA
TCTCCTTTGTTCGGGTTTCATACAAATCAACAGTCAAATAGCAATGGGAGCAAGTATCTC
TCCGGTATCTGGCTTCCATAACCCGCAATACGATATAAGTATTACCATTTGGAAGGTAAA
AAGTCTCAACCTTTATGGATGAATCAACCTGGTATCCATTTCTTGAGCTTCTCTAAAGAA
CCATCTTTTTCATGAAATTCTTGAACCTCAACATTTTGCCCTCAATGATGTTATCTTTAT
GGATATGTGAGTGTGTCTGATTAATAAAAACTGATAACTCGGGGACATTGTTCTTGTAGG
ATCCAAAAGAAGGGCACTGGTGGATGCAGTTTGGAGACGGGTATGTGTTGGGATACTGGC
CAAGCTTTCTATTCTCTTACTTAGCAGATAGTGCATCAATAGTAGAGTGGGGTGAAAAAA

Last Updated on 06.12.2018 16:11