Sequences from confirmation amplicon


Confirmation sequences for line 262G07 and hit At5g57090 are shown.
Line ID 262G07
Locus At5g57090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23104223
Primers used for PCR KP02 - 8474, show primer details
   
Sequence Name 75-K021646-022-262-G07C-8409
GenBank Accession LN513157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K021646-022-262-G07C-8409
GCCATTTACAATTGGTGGGTTATATTCTTTGGGCGAAAACAAAAGGAACGATTCCTTGAG
GAAGAGCAGCCTTCAAAACATACAATTATATATATTCTTATCAATTATATTTTTCAAAGA
AACCCAACATCACTAGCTAAAACATCAAACTGTAAGTTAGATTGAAATATATATAAGACT
AAAGAGTAATCACCTGAACGATGGCGATATGGAGGAGATCACCTCGAATACCAATTGCTA
TTGAGGTGGCTGCGATCACGGCTGGTCCAGTCAAGAACCTTACGGCCATCGCAAACCCTG
CTACTGATTTTCCGCACGCAATAATCTTTGGTTGCAATGCCATAAATAGACCTATATGTA
TCAATAATTAGCAAAAAAAAAAGCACCAAAGACTATAACTAAATATATATATGAAGGGAA
CTATACCAAGACTAAACATAGCCTTTCCAAGACCAGCA
   
Sequence Name 76-K021646-022-262-G07C-KP02
GenBank Accession LN513312 [GenBank]
Sequencing primer gene specific primer, KP02: 5'-CGATATTATCTGATGCTGGTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K021646-022-262-G07C-KP02
GTTCCCTTCAATATATATTTAGTTATAGTCTTTGGTGCTTTTTTTTTTGCTAATTATTGA
TACATATAGGTCTATTTATGGCATTGCAACCAAAGATTATTGCGTGCGGAAAATCAGTAG
CAGGGTTTGCGATGGCCGTAAGGTTCTTGACTGGACCAGCCGTGATCGCAGCCACCTCAA
TAGCAATTGGTATTCGAGGTGATCTCCTCCATATCGCCATCGTTCAGGTGATTACTCTTT
AGTCTTATATATATTTCAATCTAACTTACAGTTTGATGTTTTAGCTAGTGATGTTGGGTT
TCTTTGAAAAATATAATTGATAAGAATATATATAATTGTATGTTTTGAAGGCTGCTCTTC
CTCAAGGAATCGTTCCTTTTGTTTTCGCCAAAGAATATAACCACCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11