Sequences from confirmation amplicon


Confirmation sequences for line 336H07 and hit At5g57660 are shown.
Line ID 336H07
Locus At5g57660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23356687
Primers used for PCR 03ma - 8474, show primer details
   
Sequence Name 24-K114570-0022-336-H07C-03ma
GenBank Accession LN497097 [GenBank]
Sequencing primer gene specific primer, 03ma: 5'-TCTCCCGTTAACTAACAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114570-0022-336-H07C-03ma
TAAGCTCTCTGCTTTCACTTACCCTTCTAATCAGTCAGCCACAGTGTAATTTCCCTCCGT
CAAAATCAAATCTTTTATCATAAAATTTGCTTAAATTCAGTAAAAAACTAATCTTTGAAT
TTTGTGATTTTCAGGTTTCGACTTCTTCTATTGAATACGGTGTAGTTCCTGACGGAAACA
CAAACAACTCTGTTAACCGGAGCACGATCACTAGCTCGACGACTGGTGGTGATCATCAAG
CGAGCTCTATGGATAGAGAAGCTAGGGTTTTGAGGTACAGAGAGAAGAGAAAGAACAGGA
AATTTGAGAAGACGATTCGTTACGCTTCGAGGAAAGCTTATGCAGAGTCACGGCCAAGGA
TCAAAGGCCGGTTTGCGAAAAGAACAGAGACAGAAAACGACGACNTTTTCCTTAGTCATG
TTTATGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATATCCGCACGGTT
   
Sequence Name 11-K114569-0022-336-H07C-8409
GenBank Accession LN491219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114569-0022-336-H07C-8409
GCATTTACNATTGAGCATAAACATGACTAAGGAAAATGTCGTCGTTTTCTGTCTCTGTTC
TTTTCGCAAACCGGCCTTTGATCCTTGGCCGTGACTCTGCATAAGCTTTCCTCGAAGCGT
AACGAATCGTCTTCTCAAATTTCCTGTTCTTTCTCTTCTCTCTGTACCTCAAAACCCTAG
CTTCTCTATCCATAGAGCTCGCTTGATGATCACCACCAGTCGTCGAGCTAGTGATCGTGC
TCCGGTTAACAGAGTTGTTTGTGTTTCCGTCAGGAACTACACCGTATTCAATAGAAGAAG
TCGAAACCTGAAAATCACAAAATTCAAAGATTAGTTTTTTACTGAATTTAAGCAAATTTT
ATGATAAAAGATTTGATTTTGACGGAGGGAAATTACACTGTGGCTGACTGATTGAGAAGG
GTAAGTGAAAGCAGAGAGCTTTGATCTGCAGAAATCAATATCGAAGCAATGATCATTGTT
AGTAAACGAGGAGA

Last Updated on 06.12.2018 16:11