Sequences from confirmation amplicon


Confirmation sequences for line 714C03 and hit At5g58270 are shown.
Line ID 714C03
Locus At5g58270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23562593
Primers used for PCR ME37 - 8474, show primer details
   
Sequence Name 61-K027641-022-714-C03C-8409
GenBank Accession LN510421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K027641-022-714-C03C-8409
AGCCATTTACATTGAATATATCCTGAAACGGAATTCTGGATTGTCTCTCATCCACAAATA
GCCAGCAAGCGTACGGAGAATCTTCATGTCAGCCATAGCTGAGTCTGAATCCGACGAGGT
TGTCTTGATTTCCTTAGTCTTGGTGGTGGTTTGGTCTGGATTGGGAGTAGAAGTCGAGAA
GAGACGACCATTAAGCATTGAACTCCGTTGAACAAAACGAATTGGGGATGGTGATGGTGA
CGGGGATGGTGAGTTATCTGAGAGGAAGGCATTGATAACCGGAGATGTTCGAATTGAGTT
ACGTCTGATGTAATTGGAGAAACCATTATGAAGACGATAATCAGAGCGAAGACTGTAGGA
AAAAGATGGGATTTTGGGTTGAGGTTGAAGATTAACGCGACGAANTTAAGA
   
Sequence Name 62-K027641-022-714-C03C-ME37
GenBank Accession LN510656 [GenBank]
Sequencing primer gene specific primer, ME37: 5'-GCTCTTACTCTGTCGCGTTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K027641-022-714-C03C-ME37
TACAATCCATCTTTTCCTACAGTCTTCGCTCTGATTATCGTCTTCATAATGGTTTCTCCA
ATTACATCAGACGTAACTCAATTCGAACATCTCCGGTTATCAATGCCTTCCTCTCAGATA
ACTCACCATCCCCGTCACCATCACCATCCCCAATTCGTTTTGTTCAACGGAGTTCAATGC
TTAATGGTCGTCTCTTCTCGACTTCTACTCCCAATCCAGACCAAACCACCACCAAGACTA
AGGAAATCAAGACAACCTCGTCGGATTCAGACTCAGCTATGGCTGACATGAAGATTCTCC
GTACGCTTGCTGGCTATTTGTGGATGAGAGACAATCCAGAATTCCGTTTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACA

Last Updated on 06.12.2018 16:11