Sequences from confirmation amplicon


Confirmation sequences for line 181H08 and hit At5g60370 are shown.
Line ID 181H08
Locus At5g60370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24283532
Primers used for PCR 12cy - 8474, show primer details
   
Sequence Name 42-K109343-0022-181-H08C-12cy
GenBank Accession LN504490 [GenBank]
Sequencing primer gene specific primer, 12cy: 5'-AGGACATTAACACAAAACTCCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K109343-0022-181-H08C-12cy
ACAAATGACCAACGTTTTCCTTAACTCTATCAAACACTTACCTTCCATTCCTCCTCTGTG
GCTCAGCTGGAAGTGTATCACGGAGTCGAGTCTTTGTATCTATCAATATTGGTCCGCTAT
CAGAAGACTCTTCTGCATACGCCTTTCTAAGCTCGTCAATAATTCCCACAATCCATTGAC
CTCCAACAAATCCTAAGCTTTACAAGGTAAAATGCAACATATTTAAAGTCTCATTTGATG
TATGCTACAAAACATTATCTTGCTAGGAAAAGGTAAAAGCCACGAGAATGTTACAAAAGA
AGATCGAAACTAGAGGGGTAGAAGGAACTCACAGTAGCAATTCACGAGTTCGTCCTTCAA
ACAAGAACTGATTAACACCAGCAATGGAATTCAACAGCTTCAATGCCCACTTATCTTCAT
TTGATTCAACTTTTACTCTCACTTTTTTAACTACCTACCCAATTATTCAACTTCCCGGAC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGGTTATT
   
Sequence Name 34-K109342-0022-181-H08C-8409
GenBank Accession LN501449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K109342-0022-181-H08C-8409
ATTTACATTGAATTATCCTGTCCGGGAAGTTGAATAATTGGGTAGGTAGTTAAAAAAGTG
AGAGTAAAAGTTGAATCAAATGAAGATAAGTGGGCATTGAAGCTGTTGAATTCCATTGCT
GGTGTTAATCAGTTCTTGTTTGAAGGACGAACTCGTGAATTGCTACTGTGAGTTCCTTCT
ACCCCTCTAGTTTCGATCTTCTTTTGTAACATTCTCGTGGCTTTTACCTTTTCCTAGCAA
GATAATGTTTTGTAGCATACATCAAATGAGACTTTAAATATGTTGCATTTTACCTTGTAA
AGCTTAGGATTTGTTGGAGGTCAATGGATTGTGGGAATTATTGACGAGCTTAGAAAGGCG
TATGCAGAAGAGTCTTCTGATAGCGGACCAATATTGATAGATACAAAGACTCGACTCCGT
GATACACTTCCAGCTGAGCCACAGAGGAGGAATGGAAGGTAAGTGTTTGATAGAGTTAAG
GAAAACGTTGGTCATTTTTGGTTTTGGTTTAATACGTGGGAGTTTTGTGTNAATGTCC

Last Updated on 06.12.2018 16:11