Sequences from confirmation amplicon


Confirmation sequences for line 346B03 and hit At5g60520 are shown.
Line ID 346B03
Locus At5g60520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24332952
Primers used for PCR 27rq - 8474, show primer details
   
Sequence Name 13-K116292-0022-346-B03C-27rq
GenBank Accession LN492203 [GenBank]
Sequencing primer gene specific primer, 27rq: 5'-GCTGACTTTTCCCTGTTAGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116292-0022-346-B03C-27rq
GTCAGAACTTATATTTGAAGCTAGTGGTGGTTCTCCCAAAGAATTTAATAAACAAAGTCG
AAGCTGTTTCTAACACGGTCTTCGATTCTTCATGTGATTTTGATCAACTCTGGACCGTCA
ATTAAGCCGGTCTCTGCTGCTTACTTCCAGCCTCTCTGGTCTATAAGAACACTCCCTCCA
CTTCTATCAAATCAAAATCCACAGAAATATACAACAAAACACTGTTTCTTTCAAAACCAC
AAATCTACATGTAAATGGCTAAAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K116291-0022-346-B03C-8409
GenBank Accession LN488503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116291-0022-346-B03C-8409
GCATTTCATTGTTTAGCCATTTACATGTAGATTTGTGGTTTTGAAAGAACAGTGTTTTGT
TGTATATTTCTGTGGATTTTGATTTGATAGAAGTGGAGGGAGTGTTCTTATAGACCAGAG
AGGCTGGAAGTAAGCAGCAGAGACCGGCTTAATTGACGGTCCAGAGTTGATCAAAATCAC
ATGAAGAATCGAAGACCGTGTTAGAAACAGCTTCGACTTTGTTTATTAAATTCTTTGGGA
GAACCACCACTAGCTTCAAATATTAAGTTCTTGACATGAATAATTAACAAAAATTGAAAA
TCTGCATTTCTAACAGGAAAAAGTCAGCA

Last Updated on 06.12.2018 16:11