Sequences from confirmation amplicon


Confirmation sequences for line 556F03 and hit At5g60730 are shown.
Line ID 556F03
Locus At5g60730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24424925
Primers used for PCR 13vk - 8474, show primer details
   
Sequence Name 66-K117751-0022-556-F03C-13vk
GenBank Accession LN511697 [GenBank]
Sequencing primer gene specific primer, 13vk: 5'-ATGAGAAACCTTCAAGAACTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117751-0022-556-F03C-13vk
TTGTTAATCTCATCAGCAAGACTTTTTAGATCTTAGGACAGCAAAAGAATTTAACTTATT
GTTATGCACTGGAAACGAAAGTTGAAAAAAAAAAATCTTATTTCCAAATGAGATCACCCA
TGAACTTAAGTGCGGGAACCCCTCTTATCTCTGCGTCTAGCAATGGCGATTGGATCAATT
TTAACCCCGAAAGTTCTGTATCATTCTGAATAAGTCCAAGCACACGCGTTTGTTCCTGAA
ACATCAACAACCATATCACATTAAGTCACAAGCAATGGCTATGCCTATAGTATATACTAA
ACAGATACTGAATTAAAATCAAAAGTTTCAGTAACTTTTGCTTGCAAAGAGTTTGTAAAT
ACCTTTCGTCTTATTGAGCAAAATTTGCAATCGGATTCAAATTGAGGTAGAAGCTGATTA
ACAATAAGCCGGTGGACTGGCACATTTTCTTTTCTTAAAGATGCATGTAACCTTGAAGAC
TCGTTTATTGCCATAACCTAAAGGGAAATATAATAGCCAAGTGAAAAAGAAATGGCTTCC
AAAGAAAGAAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TA
   
Sequence Name 41-K117750-0022-556-F03C-8409
GenBank Accession LN504240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117750-0022-556-F03C-8409
TTTTCTTCTTTGGAGCCATTTCTTTTTCACTTGGCTATTATATTTCCCTTTAGGTTATGG
CAATAAACGAGTCTTCAAGGTTACATGCATCTTTAAGAAAAGAAAATGTGCCAGTCCACC
GGCTTATTGTTAATCAGCTTCTACCTCAATCTGAATCCGATTGCAAATTTTGCTCAATAA
GACGAAAGGTATCTACAAACTCTCTGCAAGCAAAAGTTACTGAAACTTTTGATTTTAATT
CAGTATCTGTTTAGTATATACTATAGGCATAGCCATTGCTTGTGACTTAATGTGATATGG
TTGTTGATGTTTCAGGAACAAACGCGTGTGCTTGGACTTATTCAGAATGATACAGAACTT
TCGGGGTTAAAATTGATCCAATCGCCATTGCTAGACGCAGAGATAAGAGGGGTTCCCGCA
CTTAAGTTCATGGGTGATCTCATTTGGAAATAAGATTTTTTTTTTTCAACTTTCGTTTCC
AGTGCATAACAATAAGTTAAATTCTTTTGCTGTCCTAAAATCTAAAAAGTCTTGCTGATG
AGATTAAACAAAATACCAATGTTATTAACTTTAAGCAAAGAAAGTAATGGAAGTTCTTGA
G

Last Updated on 06.12.2018 16:11