Sequences from confirmation amplicon


Confirmation sequences for line 100H02 and hit At5g60870 are shown.
Line ID 100H02
Locus At5g60870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24485877
Primers used for PCR MR35 - 8474, show primer details
   
Sequence Name 06-K016272-022-100-H02C-8409
GenBank Accession LN488581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K016272-022-100-H02C-8409
TTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTTAAGAAATTGACTTCGACCGGAG
TAGTTTGGATCTCAGGGAGACCTGGAACTCCTAGCTGGAAATCTTTAGCGTTTCCCCACA
TCCAGAGTTCACCCCCATCTGTCAAAAACCAATCCCATCCTTTTGGTTTTGTTTGAGTTA
TTTATCATGTCAAATGAACATATCTATAGCTAAATATACCTGTTATCGCTGCAGAAGAGG
AACCTCCACTAGCGATGAAGACTATCTTTTTGTCAGCTAATGCTTCGATTTCAGTAGGAA
CTTTCTGGTTTCGCAGCGAGGAACTACCCAACTGGCCGTGGCCACCGTGGCCCCATGAGA
GTACTTTACCTTCCTCTGTTTTCACATCAAGCAATTTTCAAG
   
Sequence Name 10-K016272-022-100-H02C-MR35
GenBank Accession LN490425 [GenBank]
Sequencing primer gene specific primer, MR35: 5'-GACTTGAAAATTGCTTGATGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K016272-022-100-H02C-mr35
GTAAAGTACTCTCATGGGGCCACGGTGGCCACGGCCAGTTGGGTAGTTCCTCGCTGCGAA
ACCAGAAAGTTCCTACTGAAATCGAAGCATTAGCTGACAAAAAGATAGTCTTCATCGCTA
GTGGAGGTTCCTCTTCTGCAGCGATAACAGGTATATTTAGCTATAGATATGTTCATTTGA
CATGATAAATAACTCAAACAAAACCAAAAGGATGGGATTGGTTTTTGACAGATGGGGGTG
AACTCTGGATGTGGGGAAACGCTAAAGATTTCCAGCTAGGAGTTCCAGGTCTCCCTGAGA
TCCAAACTACTCCGGTCGAAGTCAATTTCTTAAATGGCTTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11