Sequences from confirmation amplicon


Confirmation sequences for line 200G12 and hit At5g60910 are shown.
Line ID 200G12
Locus At5g60910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24505542
Primers used for PCR BL88 - 8474, show primer details
   
Sequence Name 54-K105140-0022-200-G12C-BL88
GenBank Accession LN508474 [GenBank]
Sequencing primer gene specific primer, BL88: 5'-GGTGAGTCCACAAAACATCATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K105140-0022-200-G12C-BL88
TATTTAAGATTCGAGTTTCCCTCCTGACTCGGTTATCAAAGCAAACGATCACTATAAATC
TCTATGATCGTACATAAAAACATATATATACACTTTCACAAAACGCATTTAAAATATCAG
TACGAACTTACATATATGTTTAACGTTATGTTATTGTCCATTGGGGATTATAGCACATCC
TTGTTATAAATGCTTTATTTCATCGTTTCAAATCAACCTTTCTTTCAAATATATGGTATA
CTCAAGTATTATTATACATGTTATCTACATACATATCTTCGTACGTGTCTGAGTGCACGT
GCTTCCATACATCAAAAGGGGCAATCAAATCGTGAGTGTGTGTACTAAGTTTCATAATTT
TATGCTATTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC
   
Sequence Name 38-K105141-0022-200-G12C-8409
GenBank Accession LN502974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105141-0022-200-G12C-8409
CATTTACAATTGAATATATCCTGAAAATAGCATAAAATTATGAAACTTAGTACACACACT
CACGATTTGATTGCCCCTTTTGATGTATGGAAGCACGTGCACTCAGACACGTACGAAGAT
ATGTATGTAGATAACATGTATAATAATACTTGAGTATACCATATATTTGAAAGAAAGGTT
GATTTGAAACGATGAAATAAAGCATTTATAACAAGGATGTGCTATAATCCCCAATGGACA
ATAACATAACGTTAAACATATATGTAAGTTCGTACTGATATTTTAAATGCGTTTTGTGAA
AGTGTATATATATGTTTTTATGTACGATCATAGAGATTTATAGTGATCGTTTGCTTTGAT
AACCGAGTCAGGAGGGAAACTCGAATCTTTAAATTGAATTTGTACGTATGTTTTCTATGA
TGT

Last Updated on 06.12.2018 16:11