Sequences from confirmation amplicon


Confirmation sequences for line 072F12 and hit At5g61010 are shown.
Line ID 072F12
Locus At5g61010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24554707
Primers used for PCR 02dh - 8474, show primer details
   
Sequence Name 19-K109642-0022-072-F12C-02dh
GenBank Accession LN494797 [GenBank]
Sequencing primer gene specific primer, 02dh: 5'-GGTTTTTACTATGTTCATGTGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K109642-0022-072-F12C-02dh
TTCCGAGATGATTGAGATTTTTTCAAGTATAGTTCTTGGAGTTAAGCTTACCTAGTAATC
ACTTTATATAACATCCCTTCGTTTACATTTGTGCTTTCACCTGGAAACACTTTAGACTTT
TCTCTCTTCTGCCGTGTGTATTTAGTTGTCTAGTCAAATTTAAGTTGAGTTTAGGCTCTA
GTCTTTGGTTTTGGTTATGGCAGAGTTTGATTCCAAGGTTCCTGTTTCCGGAATGAACAA
TCATGTCTTTGAGGCATGTCACCATGTTGTTAAGGCACTGAGGGCATCTGATTCACTGCG
GTCACCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 03-K109633-0022-072-F12C-8409
GenBank Accession LN487527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109633-0022-072-F12C-8409
CATTTACAATTGAATATATCCTGTAGGTGACCGCAGTGAATCAGATGCCCTCAGTGCCTT
AACAACATGGTGACATGCCTCAAAGACATGATTGTTCATTCCGGAAACAGGAACCTTGGA
ATCAAACTCTGCCATAACCAAAACCAAAGACTAGAGCCTAAACTCAACTTAAATTTGACT
AGACAACTAAATACACACGGCAGAAGAGAGAAAAGTCTAAAGTGTTTCCAGGTGAAAGCA
CAAATGTAAACGAAGGGATGTTATATAAAGTGATTACTAGGTAAGCTTAACTCCAAGAAC
TATACTTTGAAAAAATCTCAAGTCATCTCGGAATGAAGTAAACAGAGAGGAAGAGAAATC
CCCACATGAACATAGTAAAAAC

Last Updated on 06.12.2018 16:11